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1、中华烧伤杂志2002年2月第18卷第1期ChinJBurns,February2002,Vol.18,No.1#45##论著#大鼠烫伤后下丘脑室旁核B-内啡肽的变化及D受体的作用洪新如赵小林王成海林葆城=摘要>目的探讨大鼠烫伤后不同时间下丘脑室旁核B-内啡肽水平的变化和阿片D受体的作用。方法在大鼠背部20%体表面积Ó度烫伤后不同时间,采用放射免疫分析法测定下丘脑室旁核B-内啡肽免疫活性物质含量的变化;通过下丘脑室旁核微量注射D受体激动剂DPDPE或其拮抗剂ICI174864后心功能指标和存活时间
2、的变化,探讨D受体在大鼠烫伤休克中的作用。结果(1)大鼠烫伤后1、2、4h,下丘脑室旁核B-内啡肽免疫活性物质的含量显著升高(P<0.01);(2)与对照组比较,给予ICI174864后不同时间,动物平均动脉压、左心室内压最大上升速率与各自伤前基础值的比值显著升高,给予DPDPE则显著降低。但无论给予ICI174864或DPDPE,心率比值的变化相差不显著;(3)ICI174864组动物的平均存活时间明显长于DPDPE组(P<0.01)。结论下丘脑室旁核B-内啡肽的过量增加是促使休克加重和加快死
3、亡的原因之一,该处D受体可能起了重要的介导作用;拮抗D受体的作用有利于对抗烫伤休克,延长存活时间。=关键词>B-内啡肽;D受体;室旁核;烫伤;大鼠,SDThepostburnchangeinhypothalamicparaventricularB-endorphinandtherolesofD-receptorinscaldedratsHONGXinru,ZHAOXiaolin,WANGChenghai,LINBaocheng.DepartmentofNeurobiology,TheSecond
4、MililtaryMedicalUniversity,Shanghai200433,P.R.China=Abstract>ObjectiveToinvestigatethepostburnchangeinhypothalamicparaventricularB-endor-phinandtherolesofD-receptorinscaldedrats.MethodsMaleSprague-Dawley(SD)ratswererandomlydividedinto3groups,i.e.ICI1
5、74864,DPDPEandcontrolgroups.Theratswereinflictedwith20%TB-SAofÓdegreescaldingonthebackbyboiling(100e)water.ThepostburnchangeinthetissuecontentofthehypothalamicparaventricularB-endorphinwasdeterminedbyradioimmunoassay(RIA).TheeffectsofD-receptorinscal
6、dedshockratswereinvestigatedbyobservingthechangeoftheratscsurvivaltimeandcardiacindicesafterthemicro-injectionofD-receptoragonistDPDPEorantagonistICI174864intothehy-pothalamicparaventricle.Results(1)ThetissuecontentofthehypothalamicparaventricularB-e
7、ndor-phinincreasedsignificantly(P<0.01)at1,2and4postburnhours(PBHs)inthescaldedrats.(2)Whencomparedwiththatofcontrolgroup,theratioofthecardiovascularparameters[meanarterialpressure(MAP),dp/dtmaxandHR]wereobviouslyincreasedatdifferenttimepointsinratsw
8、ithpre-injectionofICI174864whereastheratiowasdecreasedwhenDPDPEwasused.Nevertheless,thechangeintheheartrateratiowasnotobviouswhetherICI174864orDPDPEwasused.(3)TheaverageanimalsurvivaltimeinICI174864groupwasmuchlongerthanthatinDPDPEgroup.ConclusionAne
