蛋白质生化分离技术

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1、BioseparationofproteinsChapter1Introduction1IntroductionBiomaterialsolutionproperties:(1)Dilutesolution(2)Componentscomplicate(3)Unstable(4)BatchesdifferenceBioseparationrequirements(1)Highpurity(2)Largescale(3)Economical(4)BiocompatibleNewwordsandvocabulary1cellfusion2interestprotein3bioc

2、ompatible4purification5biologicalmaterial6plantcellculture7fermentation8strategiesIITheNeedforbioseparationProcessrequirements(1)Safety(2)Quality(3)Amount(4)PreventingPosttranslationalchange(5)LowcostWhatwillbioseparationdo?(1)Modificationofexistingprocedures(2)Developingnewandnovelbiosepa

3、rationtechnologyDifficultyofgeneralizedalgorithmprocessesandsubtledifferenceofdifferentproteinsNewwordsandvocabularyProtocol(方案）Algorithm(法则）Generalized(通用的，一般化的）Invaluable(有价值的）Inactivate(失活的）Therapeutic(治疗的)Viable(可行的）IIIClassificationofbioseparationsteps(1)Bioseparationprocedures(1)Remo

4、valofinsolubles(2)Isolationofproduct(3)Purification(4)PolishingIIIClassificationofbioseparationsteps(2)Bioseparationunitoperation(1)Celldisruption(2)Centrifugation(3)Chromatography(4)Drying(5)Evaporation(6)Extraction(7)Filtration(8)Membraneseparation(9)PrecipitationIIIClassificationofbiose

5、parationsteps(3)Qualityrequirements:Productsfreefrom:(1)detergent(2)endotoxins(3)proteases(4)immunogenicsubstance(5)microbiologicalcontamination(6)modifiedproteins(7)nucleidacids(8)oligonucleotide(9)enterotoxinsIIIClassificationofbioseparationsteps(4)Therapeuticusagerequirements:(1)Potency

6、(2)Identity(3)Abnormaltoxicity(4)Nucleicacids(5)Homogeneity(6)AbsenceofconflictingactivityProcessrequirements:(1)Maximizeproductyieldininitialstep(2)Limitthenumberofsteps(3)IntensificationIIIClassificationofbioseparationsteps(5)NewwordsandvocabularyHigh-resolutionfractionaton(高分辩率分级）Harves

7、ting(收获，收割）Clarification（澄清）Enrichment（富集）Hazardous（危险）Integrate（集成）Intensification（集成，加强）Expandedbedadsorption（扩张床）Homogenate（匀浆液）Fermentationbroth（发酵液）IVUpstreamanddownstreamprocessing(1)Minimizelossofthevaluablebiologicalproduct(1)Locationandformofproteinsi