资源描述:
《幽门螺杆菌培养及抗原蛋白的分离纯化》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、ISSN1009-3079CN14-1260/R世界华人消化杂志2008年4月8日;16(10):1098-1104文献综述REVIEW幽门螺杆菌培养及抗原蛋白的分离纯化赵圣国,王加启,刘光磊,程金波,张春刚 赵圣国,王加启,刘光磊,程金波,张春刚,中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室北京市100094国家“十一五”科技攻关奶业重大专项资助项目,No.2006BAD04A03作者贡献分布:赵圣国对此文做主要贡献;此课题由王加启提出设计;刘光磊,程金波和张春刚对本文修改和审阅;文章思路及写作由赵圣国完成.通讯作者:
2、王加启,100094,北京市圆明园西路2号,中国农科院北京畜牧兽医研究所.wang-jia-qi@263.net电话:010-62810458收稿日期:2007-09-13修回日期:2008-02-27CultureofHelicobacterpyloriandpurificationofantigenproteinSheng-GuoZhao,Jia-QiWang,Guang-LeiLiu,Jin-BoCheng,Chun-GangZhangSheng-GuoZhao,Jia-QiWang,Guang-LeiLiu,Jin-BoCh
3、eng,Chun-GangZhang,StateKeyLaboratoryofAnimalNutrition,InstituteofAnimalScience,ChineseAcademyofAgricultureSciences,Beijing100094,ChinaSupportedby:theDairyMomentousProjectofNationalKeyTechnologyR&DProgramduringthe11thFive-YearPlanPeriod,No.2006BAD04A03Correspondenceto:
4、Jia-QiWang,InstituteofAnimalScience,ChineseAcademyofAgriculturalScience,2YuanmingyuanWesternRoad,Beijing100094,China.wang-jia-qi@263.netReceived:2007-09-13Revised:2008-02-27AbstractHelicobacterpylori(Hpylori)liveinthestomachofhuman.Itisakindofcurvebacteriawhichcanleadt
5、ochronicgastritis,pepticulcerandgastriccancer.Usually,antibioticsareusedtotreatthepatients,buttheyhavealotofsideeffects.MilkofmilchcowimmunizedwithHpylorivaccinecanpreventandtreattheinfectionofHpyloriwithoutsideeffects.Duringtheproductionofimmunemilk,thebasicworkistopr
6、eparevaccine.Duringthepreparationofsub-unitvaccine,keyistoisolateandpurifytheantigenprotein.Inthisarticle,wediscussthecultureofHpylori(solidandliquidculture),therecombinantexpressionandpurificationofitsantigenprotein(urease,VacA,CagAandlipopolysaccharide).KeyWords:Heli
7、cobacterpylori;Culture;Urease;VacuolizationcytotoxinA;CytotoxinassociatedantigenA;LipopolysaccharidesZhaoSG,WangJQ,LiuGL,ChengJB,ZhangCG.CultureofHelicobacterpyloriandpurificationofantigenprotein.ShijieHuarenXiaohuaZazhi2008;16(10):1098-1104摘要幽门螺杆菌(Hpylori)是一种生存于人胃中的弯曲
8、状菌,能导致人的慢性胃炎,消化性溃疡和胃癌,其治疗多用抗生素治疗,但其副作用较大.给奶牛免疫Hpylori疫苗后,其分泌的牛奶中含抗Hpylori的免疫球蛋白,能够预防和治疗人类Hpylori感染,且没副作用.在生产免疫乳时,Hpyl