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1、辣螺糖蛋白的提取、分离纯化及理化性质分析AdvancesinMarineSciences海洋科学前沿,2014,1,1-8PublishedOnlineSeptember2014inHans.http://www.hanspub.org/journal/amshttp://dx.doi.org/10.12677/ams.2014.11001StudyonExtraction,Isolation,PurificationandPhysicalChemistryPropertiesoftheGlycoproteinfromThaisclavigeraKusterHejunCai,FeifeiGe,
2、BimYu,PanZhao,YouleQu*CollegeofFoodandPharmaceuticalScience,ZhejiangOceanUniversity,Zhoushan*Email:youle1960@sina.comReceived:Jul.11,2014;revised:Jul.26,2014;accepted:Aug.4,2014Copyright?2014byauthorsandHansPublishersInc.ThisworkislicensedundertheCreativeCommonsAttributionInternationalLicense(CCBY).
3、辣螺糖蛋白的提取、分离纯化及理化性质分析AdvancesinMarineSciences海洋科学前沿,2014,1,1-8PublishedOnlineSeptember2014inHans.http://www.hanspub.org/journal/amshttp://dx.doi.org/10.12677/ams.2014.11001StudyonExtraction,Isolation,PurificationandPhysicalChemistryPropertiesoftheGlycoproteinfromThaisclavigeraKusterHejunCai,FeifeiGe,
4、BimYu,PanZhao,YouleQu*CollegeofFoodandPharmaceuticalScience,ZhejiangOceanUniversity,Zhoushan*Email:youle1960@sina.comReceived:Jul.11,2014;revised:Jul.26,2014;accepted:Aug.4,2014Copyright?2014byauthorsandHansPublishersInc.ThisworkislicensedundertheCreativeCommonsAttributionInternationalLicense(CCBY).
5、http://creativecommons.org/licenses/by/4.0/thththAbstractPurpose:Thispaperstudiedonextraction,isolationandpurificationaswellasphysicalchemistrypropertiesoftheGlycoproteinfromThaisclavigeraKuster.Method:Thecrudeextractwasthenfractionatedwith3concentrationsofammoniumsulfatesolution.LGP-1,LGP-2,LGP-3,L
6、GP-4werepreparedfromthecrudethroughDEAE-cellulose.LGP-I,LGP-II,LGP-III,LGP-IV,LGP-V,LGP-VI,LGP-VIIwerepreparedthroughSephadexG-100chromatography.Result:Theresultsshowedthatthreeglycoproteincomponentswerecarriedoutfrom40%gradeammoniumsulphateprecipitation,namedLGP-I,LGP-II,LGP-III.Threepeaksappearedf
7、or60%s,namedLGP-IV,LGP-VandLGP-VI.Thesugarwere17.86%,20.00%,13.14%,21.57%,andthecontentsofproteinwere56.72%,62.58%,71.86%,60.86%.TheIRindicatedglycoproteingroupscontained?OH,?COO?,?NH,?C?O?Ccharacteri
