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ID:37416925
大小:2.84 MB
页数:40页
时间:2019-05-23
《三术克异汤治疗子宫内膜异位症的实验研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、中文摘要三术克异汤治疗子宫内膜异位症的实验研究目的:通过建立子宫内膜异位症大鼠模型探讨中药三术克异汤对子宫内膜异位症的治疗作用及作用机制。方法:将健康雌性未孕Wistar大鼠70只(2月龄)随机选出16只作为正常对照组和假手术组(每组各8只)外,其余大鼠均采用自体内膜移植法建立子宫内膜异位症大鼠模型,假手术组在相应位置移植脂肪组织。将成模大鼠48只随机分组,模型对照组8只,中药低、中、高剂量组各为10只,达那唑组10只。按10ml/kg给中药组灌胃,每日1次,连续4周(每周休息一日)。达那唑用法与中药组相同,其余三组灌服等量的蒸馏水。灌胃给药4周后,腹主动脉取血,分别进
2、行血液流变性和血清性激素检测,取腹腔液进行癌标记抗原CAl25的检测,并对各组的异位病灶体积进行比较,剥离种植的异位病灶及相应的一段在位子宫内膜制作标本,进行HE染色,对异位病灶进行原位细胞凋亡检测,观察三术克异汤对异位病灶病理组织学和细胞凋亡的影响。结果:(1)模型对照组的血液流变性异常,经三术克异汤和达那唑治疗后得到改善,其中尤以三术克异汤中剂量组最为明显,主要是全血粘度(高、中、低切)和全血还原粘度(高、中切)的降低,与模型对照组相比均具有极显著性差异(P3、组存在血清激素水平的紊乱,雌、孕激素和催乳素水平高于正常对照组,用三术克异汤治疗后,尤其是中剂量组对激素水平的调节优于达那唑组,达那唑组的血清雄激素水平升高,血清卵泡刺激素与黄体生成素无明显变化。(3)模型对照组CAl25水平高于正常对照组(P<0.01),用药后各组CAl25均降低(P<0.01),尤以中药中剂量组降低优于达那唑组(P<0.05)。(4)用药组异位病灶萎缩,其中三术克异汤中剂量、高剂量及达那唑组的异位病灶体积小于三术克异汤低剂量组,尤以中药中剂量组最为明显,差别有极显著性意义(P<0.01)。(5)组织病理学观察发现模型对照组的异位内膜生长良好,三术克4、异汤治疗组的病灶有萎缩性改变,和达那唑组相似,对在位内膜无明显影响。(6)应用TUNEL法观察细胞凋亡指数发现,三术克异汤可促进异位内膜细胞凋亡,中剂量组优于低剂量与高剂量组(P5、rimentalStudyoftheeffectofSanzhukeyitangonEndometriosislAbstract]objectlve:TostudytheeffectofSanzhukeyitangonendometriosisintheratmodelandinvestigatethemechanism.Method:70virgin、female、healtyWistarratsandaged2monthswereinvolvedinthestudy.16ratsweredividedintotwogroups:normalcontrolgroup(6、n=8),shamendometriosisgmu“n=8).Otherratswereusedintheexperimentalinductionofendomctriosisbyautologusendometrialsegmentsimplanmdintheperitoneumandmesometriumofrats.48ratswithsuccessfulexperimentalendometriosiswererandomlydividedintofivegroups:Modelcontrolg∞up(n=8),Dan口.olgroup(n=lo)andSan7、zhukeyitanggroupsincludingthl^eedoses(10w,medium,high,respecmllyn=lO),Dallyadministrationofdanazolorherbdecoctionordistilledwaterwas宙venbygastricgavagerespectivelyforfourweeks.TheeffectsofChinesedrugsonbloodrheology,serumsexhormone,CAl25,histopathologicexaminationandapopt
3、组存在血清激素水平的紊乱,雌、孕激素和催乳素水平高于正常对照组,用三术克异汤治疗后,尤其是中剂量组对激素水平的调节优于达那唑组,达那唑组的血清雄激素水平升高,血清卵泡刺激素与黄体生成素无明显变化。(3)模型对照组CAl25水平高于正常对照组(P<0.01),用药后各组CAl25均降低(P<0.01),尤以中药中剂量组降低优于达那唑组(P<0.05)。(4)用药组异位病灶萎缩,其中三术克异汤中剂量、高剂量及达那唑组的异位病灶体积小于三术克异汤低剂量组,尤以中药中剂量组最为明显,差别有极显著性意义(P<0.01)。(5)组织病理学观察发现模型对照组的异位内膜生长良好,三术克
4、异汤治疗组的病灶有萎缩性改变,和达那唑组相似,对在位内膜无明显影响。(6)应用TUNEL法观察细胞凋亡指数发现,三术克异汤可促进异位内膜细胞凋亡,中剂量组优于低剂量与高剂量组(P5、rimentalStudyoftheeffectofSanzhukeyitangonEndometriosislAbstract]objectlve:TostudytheeffectofSanzhukeyitangonendometriosisintheratmodelandinvestigatethemechanism.Method:70virgin、female、healtyWistarratsandaged2monthswereinvolvedinthestudy.16ratsweredividedintotwogroups:normalcontrolgroup(6、n=8),shamendometriosisgmu“n=8).Otherratswereusedintheexperimentalinductionofendomctriosisbyautologusendometrialsegmentsimplanmdintheperitoneumandmesometriumofrats.48ratswithsuccessfulexperimentalendometriosiswererandomlydividedintofivegroups:Modelcontrolg∞up(n=8),Dan口.olgroup(n=lo)andSan7、zhukeyitanggroupsincludingthl^eedoses(10w,medium,high,respecmllyn=lO),Dallyadministrationofdanazolorherbdecoctionordistilledwaterwas宙venbygastricgavagerespectivelyforfourweeks.TheeffectsofChinesedrugsonbloodrheology,serumsexhormone,CAl25,histopathologicexaminationandapopt
5、rimentalStudyoftheeffectofSanzhukeyitangonEndometriosislAbstract]objectlve:TostudytheeffectofSanzhukeyitangonendometriosisintheratmodelandinvestigatethemechanism.Method:70virgin、female、healtyWistarratsandaged2monthswereinvolvedinthestudy.16ratsweredividedintotwogroups:normalcontrolgroup(
6、n=8),shamendometriosisgmu“n=8).Otherratswereusedintheexperimentalinductionofendomctriosisbyautologusendometrialsegmentsimplanmdintheperitoneumandmesometriumofrats.48ratswithsuccessfulexperimentalendometriosiswererandomlydividedintofivegroups:Modelcontrolg∞up(n=8),Dan口.olgroup(n=lo)andSan
7、zhukeyitanggroupsincludingthl^eedoses(10w,medium,high,respecmllyn=lO),Dallyadministrationofdanazolorherbdecoctionordistilledwaterwas宙venbygastricgavagerespectivelyforfourweeks.TheeffectsofChinesedrugsonbloodrheology,serumsexhormone,CAl25,histopathologicexaminationandapopt
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