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1、入门指南等位基因鉴别实验简介和示例实验美国应用生物系统公司7300/7500实时定量PCR仪设计等位基因鉴别实验设定反应板读取等位基因鉴别实验扩增前信号生成扩增数据读取等位基因鉴别实验扩增后信号©Copyright2004,AppliedBiosystems.Allrightsreserved.ForInternalUseOnly.ForResearchUseOnly.Notforuseindiagnosticprocedures.AuthorizedThermalCyclerThisinstrument,SerialNo___________,isanAu
2、thorizedThermalCycler.Itspurchasepriceincludestheup-frontfeecomponentofalicenseunderUnitedStatesPatentNos.4,683,195,4,683,202and4,965,188,ownedbyRocheMolecularSystems,Inc.,andundercorrespondingclaimsinpatentsoutsidetheUnitedStates,ownedbyF.Hoffmann-LaRocheLtd,coveringthePolymeraseC
3、hainReaction("PCR")processtopracticethePCRprocessforinternalresearchanddevelopmentusingthisinstrument.TherunningroyaltycomponentofthatlicensemaybepurchasedfromAppliedBiosystemsorobtainedbypurchasingAuthorizedReagents.ThisinstrumentisalsoanAuthorizedThermalCyclerforusewithapplicatio
4、nslicensesavailablefromAppliedBiosystems.ItsusewithAuthorizedReagentsalsoprovidesalimitedPCRlicenseinaccordancewiththelabelrightsaccompanyingsuchreagents.PurchaseofthisproductdoesnotitselfconveytothepurchaseracompletelicenseorrighttoperformthePCRprocess.Furtherinformationonpurchasi
5、nglicensestopracticethePCRprocessmaybeobtainedbycontactingtheDirectorofLicensingatAppliedBiosystems,850LincolnCentreDrive,FosterCity,California94404.DISCLAIMEROFLICENSE:Norightsforanyapplication,includinganyinvitrodiagnosticapplication,areconveyedexpressly,byimplicationorbyestoppel
6、underanypatentorpatentapplicationsclaiminghomogeneousorreal-timedetectionmethods,includingpatentscoveringsuchmethodsusedinconjunctionwiththePCRprocessorotheramplificationprocesses.The5'nucleasedetectionassayandcertainotherhomogeneousorreal-timeamplificationanddetectionmethodsarecov
7、eredbyUnitedStatesPatentNos.5,210,015,5,487,972,5,804,375and5,994,056,ownedbyRocheMolecularSystems,Inc.;bycorrespondingpatentsandpatentapplicationsoutsidetheUnitedStates,ownedbyF.Hoffmann-LaRocheLtd;andbyUnitedStatesPatentNos.5,538,848and6,030,787,andcorrespondingpatentsandpatentap
8、plicationsoutsidetheUnited