上海中医药大学中医学综合实验课件 小鼠辨证方法

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1、小鼠辨证方法方肇勤2011-2-171一、常规辨证2某荷瘤小鼠证候表现：腋下可见肿瘤，眯眼，消瘦，少动，爪淡白且瘦，爪和尾凉，体温略低。能不能辨证？辨证为什么？3该荷瘤小鼠证候类似于人的：腋下肿瘤，畏寒（体表温度降低往往表现为畏寒），精神不振、消瘦，舌、面部和手掌（小鼠为爪）淡白、消瘦。证候分析：肿瘤以其肿块固定不移、局部疼痛不适、发展快、预后差，往往辨证为瘀毒，即血瘀、热毒、痰凝；消瘦（类似的有脉细、舌体瘦小）为阴虚（如果见有舌红、发热、脉数为阴虚火旺，而同时出现阳虚证时，这样的火旺证候往往不明显，仅见消瘦、脉细、舌体瘦小、苔剥等）；面色淡白、畏寒、精神不振为阳虚。因此，以上

2、小鼠可以辨证为“肿瘤瘀毒，肾阴阳两虚”。4二、计量辨证原始数据和运算在Excel表中进行：5某虚证实验计量化辨证的实例：（1）检测到的原始数据序号组别编号体重腋温水平垂直爪R爪G爪B尾R尾G尾B1正常组N137.7438.11410139.4576.3182.97151.92106.28108.052正常组N233.7937.924145.5577.8685.1155.55110.91110.823正常组N334.4238.3118156.6191.2297.72158.15111.04112.294正常组N430.7938.71510145.2976.2784.31152.4

3、3100.62102.755正常组N532.1637.9228153.570.8680.16152.73110.48110.16正常组N635.8338.12310159.2187.0294.18151.38109.7109.97模型组B123.9233.101139.42107.4692.93151.74125.16110.818模型组B225.5334.302154.2811599.46149.48122.35108.119模型组B325.0134.314161.55120.57107.01157.44129.19115.8710模型组B421.8733.105160.11

4、126.49108.56149.99126.5112.5311模型组B524.0232.912158.88124.39106.99151.4124.02109.4112模型组B626.7234.434159.07119.23104.48149.4121.46109.713模型组B724.6933.372154.21118.72102.14149.79123.62108.986（2）求出爪尾r值：r=R/(R+G+B)序号组别编号爪R爪G爪B爪r尾R尾G尾B尾r1正常组N1139.4576.3182.970.467151.92106.28108.050.4152N2145.557

5、7.8685.10.472155.55110.91110.820.4123N3156.6191.2297.720.453158.15111.04112.290.4154N4145.2976.2784.310.475152.43100.62102.750.4285N5153.570.8680.160.504152.73110.48110.10.4096N6159.2187.0294.180.468151.38109.7109.90.4087模型组B1139.42107.4692.930.410151.74125.16110.810.3918B2154.2811599.460.41

6、8149.48122.35108.110.3939B3161.55120.57107.010.415157.44129.19115.870.39110B4160.11126.49108.560.405149.99126.5112.530.38611B5158.88124.39106.990.407151.4124.02109.410.39312B6159.07119.23104.480.416149.4121.46109.70.39313B7154.21118.72102.140.411149.79123.62108.980.3927（3）求出各组各检测值均数序号组别编号体重腋

7、温水平垂直爪r尾r1正常组正常137.7438.114100.4670.4152正常233.7937.9240.4720.4123正常334.4238.31180.4530.4154正常430.7938.715100.4750.4285正常532.1637.92280.5040.4096正常635.8338.123100.4680.408均数=34.12238.16714.5008.3330.4730.4157模型组虚证123.9233.1010.4100.3918虚证225.5334.30