牛磺酸对家兔钝性心肌损伤的防治作用

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1、遵义医学院硕士学位论文牛磺酸对家兔钝性心肌损伤的防治作用姓名：徐刚申请学位级别：硕士专业：病理学指导教师：邓飞;牛义民2003.6.20!些堕旦箜兰旦堡主堕塞兰望些堡皇——牛磺酸对家兔钝性心肌损伤防浩作用的实验研究摘要目的探讨牛磺酸对钝性心肌损伤的防治作用机制。材料与方法将健康中国家兔24只随机分为三组：对照组，心肌挫伤组和牛磺酸治疗组。利用B／M—II型水平式生物撞击机撞击兔胸前区制备钝性一山肌损伤模型。三组动物分别于撞击前lO分钟、撞击后30rain、2h、4h、8h及24h自股静脉采血检测心肌肌钙蛋白I(car

2、diactroponin，cTnI)与磷酸肌酸激酶(creatinephosphatekinase，CPK)I撞击后24h各组分别取6只兔检测心肌组织Ca2+_ATP酶；实验结束后分别对各组兔心进行光镜和电镜观察。结果与对照组比较，家兔被撞击后2hcTnI开始出现在血清中，且持续升高，至伤后24h仍明显高于撞击前水平，同时血清CPK活性自撞击后2h至伤后24h均明显高于撞击前水平(P

3、与4h的反应与挫伤组比较无明显区别，但8h与24hcTnI反应不如挫伤组强烈；同时CPK伤后各时点水平明显低于挫伤组(P<0．05或P<0．001)；而Ca2+．ATP酶活性明显升高(P

4、P酶垫里旦堕量篓堕堡兰!苎竺望些堕塞!堡墼翌塞叁矍壁：生里塑堕堕堕堡璺塑壅竺塑塞AstudyforeffectoftaurineOnmyocardialbluntinjuryinrabbitsAbstractObieetiveToexplore‘themechanismandeffectoftaarineonmyocardialbluntinjury．MaterialsandMethods24Chinawhiterabbitswererandomlydi讲dedintomreegroups：cona'olgroup．

5、myocardialcontusiongroup(MCgroup)andtaurinetreatedgroup(Taugroup)．WeusedBIM·ⅡHorizontalBio·impactMachinetoproducethemodelofMCinrabbits．At10thminutebeforeimpactand30thminute、2th、4th、8thand24血hourafterimpact．rabbitsbloodineachgroupweretakenfi'omfemoralveint0measa

6、ll宅theconexmtrationsofserummyocardiactropnionI(cTnI)andcreatinephosphatekila．丛e(CPK)．Attheendofexperiment(24thhourafterimpact)myocardialdssueswcⅢ-etakenoutfromthoraciccavinesofsixrabbitsineachgrouptobebuiltI

7、p10％myocardialtissuesplasmatomea．．目tlretheactivityofc

8、aa+-ATPase，others2heartsoftheexperimentalrabbitsineachgrouphadopticalandeleetronmicmscopeexaminationrespectivelytOobservemo咖lo酉caIchangesofmyocardialtissues．Results(1)The鲥强umparametersabovehadnosignificantdifferenceafterMCincomparisonwilhthoseofpre-impactinthee

9、ontro

10、groul妒>0．05)．∞mtheMCgroup，theconcentrationof8erumcTnlshowedain蝴ingtendencysince2hafterimpact,itw雏higherthanthatofpre-impactat24hafterimlmeCTheconcenWationof靶nlmCPKwas4