资源描述：
《luripotency of Embryonic Stem Cells：胚胎干细胞的多能性》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。
1、PluripotencyofEmbryonicStemCellsJayantiTokas1,PuneetTokas2,ShailiniJain3,HariomYadav31DepartmentofBiotechnology,JMIT,Radaur,Haryana,India2KITM,Kurukshetra,Haryana,India3NIDDK,NationalInstituteofHealth,Bethesda,MD20892,USAEmail:yadavhariom@gmail.comStem
2、CellsStemcellsaremastercellswithtwoimportantcharacteristicsUnspecializedcellscapableoftheirownrenewalAbilitytodifferentiateintodifferentcelltypesThestemcellsmayhavevariousdifferentiationpotentialsTotipotentPluripotentMultipotentUnipotentPluripotentStem
3、CellsPluriderivedfromlatinwordplures–meansseveralormanyMostcommonlythetermisusedtodescribestemcellsthatgiverisetocellsderivedfromallthreeembryonicgermPluripotentStemCellsEmbryonicstemcellsEmbryoniccarcinomacellsEmbryonicgermcellsTypesofPluripotentStemC
4、ellsAbnormalkaryotypes,GermlinetransmissionunlikelyUndergospontaneousdifferentiationUnabletosupportnormaldevelopmentduetoepigeneticmodificationsduringPGCsPluripotentcellsPartiallyunderstood GreatpotentialPrimordialgermCellsBlastocystFetusAdultZygoteTer
5、atomasInnercellmass(ICM)EmbryonicStemCellsEmbryonicGermCellsEmbryonicCarcinomaCellsOriginsofpluripotentcellsImmortalityUndifferentiationClonalityBroaddevelopmentalpotentialCriteriaforpluripotencyDemonstrationofpluripotencyInvitroDifferentiationtriggere
6、dwhengrowninsuspension,EmbryoidBodyformationb)Differentcellsobtainedspontaneouslyc)SpecificgrowthfactorscanbeusedtodirectthedifferentiationofEScellsintospecificcellsa)Teratomaformationwheninjectedintonudemouseb)Wheninjectedintohostblastocysts,theEScell
7、sintegrate,proliferateanddifferentiateintoallgermlineagesincludinggermcellsInvivoRegulationofpluripotencyinEScells“PluripotencyismaintainedbypromotionofproliferationandInhibitingdifferentiation”RequireotherprocessesESCellspluripotencyTranscriptionFacto
8、rsEpigeneticmodificationsStagespecificfunctioningTargetgenes/receptorsrequiredFactorsRequiredNanogOct3/4Sox2LIFc-MycKlf4Zic3CoreregulatorycircuitryinEScellsActivationoftargetgenesNanogTranscriptionfactorcontaininghomeoboxdomainDownstrea
