猪卵母细胞体外成熟的研究

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1、广西大学硕士学位论文猪卵母细胞体外成熟的研究姓名：亨利申请学位级别：硕士专业：动物遗传繁殖指导教师：石德顺2002.5.1／jj；．5gf≯确参节支仍勿c涉印卜争一卺渺75够多阳{p缈色厶々々舛信彳香(7vF)、，『亨舛谨鲁(，zL／c，)及彳乍乏7I砍肇r哗痧工V卜1／>“2衙’5芋篷·哆j劫另幻缪甲彩主锄色"Jf-#t诫弛。嚎々仑乃’中‘％。荪，沽叼：丝咝俐乏彳毡蟹罄型c工V7二下矿M!坐!堡!!!!!!!呈∑!塑丛!!!!!堕!璺竺!些堡!些Q竺垡生!!翌呈!!!!!!：墅!丛!璺垫Prefac

2、eTheavailabilityofinvitroproduction(1W)techniquesforbovineembryoshasadvancedtheunderstandingofgametephysiologyandcontributedtothecommercializationofbovineembryosproduction．Thesetechniqueshavenotbeendevelopedtosuitablelevelsinswine．Theincreasedinterestin

3、usingswineasamodelsysteminbiotechnologyapplicationandresearchareasuchasproductionoftransgenicoffsprings，spermsexingandmnitiplicationinvitrooflinesofsuperioroffspring，necessitatesincreasedfocusonimprovingtheIVM／IVF／1CMsystems．Thus，apracticalIVPsystemrequ

4、irestheimprovementsinmaturationandinfertilizationconditionstoovercomesomelimitations，whichleadtohighmaturationratesandbettermalepronucleusformation．Theembryonicdevelopmentalpro盯ammeiscodedinthemRNAandribosomesthatareaccumulatedinthecytoplasmoftheOOcyted

5、uringthefmalstageofitsmaturation．Thenumberandqualityofoocytes，whichsuccessfullyenterthereproductiveprocessdependsontheefficiencyoftheinvitroOOcytematurationconditions．ThUS，theinvestigationofinvitrooocytematurationisregardedasoneoftheprincipalwaysofincre

6、asingembryoyieldandquality．ThemajorlimitationsencounteredinIVFsystemsofporcineoocytesarepolyspermy，malepronucleusformationandlowcryo—resistanceofboargametes．Althoughtheuseofco—incubationofspermatozoawithoviductalepitherialcells．oviductalfluidandpre—incu

7、bationwithalowconcentrationofporcinefullicularfluidtoimprovefertilization，theincidenceofpolyspermyisstillhigher．Afterincorporationofthespermnucleusintotheoocytecytoplasm，somemolecularchangesareundertakenandledtotheformationofmalepronucleus．Asynchronousf

8、ormationbetweenmaleandfemalepronucleicantakeplaceifmaturationwasinadequate．Althoughtheuseoforganculture，oviductal，follicularfluidsupplementationandmodificationofsimplemedia，thedevelopmenttotheblastocyststagesisstillhamperedbythel