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ID:36661673
大小:1.09 MB
页数:32页
时间:2019-05-13
《猪卵母细胞体外成熟的研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、广西大学硕士学位论文猪卵母细胞体外成熟的研究姓名:亨利申请学位级别:硕士专业:动物遗传繁殖指导教师:石德顺2002.5.1/jj;.5gf≯确参节支仍勿c涉印卜争一卺渺75够多阳{p缈色厶々々舛信彳香(7vF)、,『亨舛谨鲁(,zL/c,)及彳乍乏7I砍肇r哗痧工V卜1/>“2衙’5芋篷·哆j劫另幻缪甲彩主锄色"Jf-#t诫弛。嚎々仑乃’中‘%。荪,沽叼:丝咝俐乏彳毡蟹罄型c工V7二下矿M!坐!堡!!!!!!!呈∑!塑丛!!!!!堕!璺竺!些堡!些Q竺垡生!!翌呈!!!!!!:墅!丛!璺垫Prefac
2、eTheavailabilityofinvitroproduction(1W)techniquesforbovineembryoshasadvancedtheunderstandingofgametephysiologyandcontributedtothecommercializationofbovineembryosproduction.Thesetechniqueshavenotbeendevelopedtosuitablelevelsinswine.Theincreasedinterestin
3、usingswineasamodelsysteminbiotechnologyapplicationandresearchareasuchasproductionoftransgenicoffsprings,spermsexingandmnitiplicationinvitrooflinesofsuperioroffspring,necessitatesincreasedfocusonimprovingtheIVM/IVF/1CMsystems.Thus,apracticalIVPsystemrequ
4、irestheimprovementsinmaturationandinfertilizationconditionstoovercomesomelimitations,whichleadtohighmaturationratesandbettermalepronucleusformation.Theembryonicdevelopmentalpro盯ammeiscodedinthemRNAandribosomesthatareaccumulatedinthecytoplasmoftheOOcyted
5、uringthefmalstageofitsmaturation.Thenumberandqualityofoocytes,whichsuccessfullyenterthereproductiveprocessdependsontheefficiencyoftheinvitroOOcytematurationconditions.ThUS,theinvestigationofinvitrooocytematurationisregardedasoneoftheprincipalwaysofincre
6、asingembryoyieldandquality.ThemajorlimitationsencounteredinIVFsystemsofporcineoocytesarepolyspermy,malepronucleusformationandlowcryo—resistanceofboargametes.Althoughtheuseofco—incubationofspermatozoawithoviductalepitherialcells.oviductalfluidandpre—incu
7、bationwithalowconcentrationofporcinefullicularfluidtoimprovefertilization,theincidenceofpolyspermyisstillhigher.Afterincorporationofthespermnucleusintotheoocytecytoplasm,somemolecularchangesareundertakenandledtotheformationofmalepronucleus.Asynchronousf
8、ormationbetweenmaleandfemalepronucleicantakeplaceifmaturationwasinadequate.Althoughtheuseoforganculture,oviductal,follicularfluidsupplementationandmodificationofsimplemedia,thedevelopmenttotheblastocyststagesisstillhamperedbythel
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