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1、OpenJournalofNatureScience自然科学,2017,5(2),203-210PublishedOnlineMay2017inHans.http://www.hanspub.org/journal/ojnshttps://doi.org/10.12677/ojns.2017.52027IdentificationandSequenceAnalysisofaCalmodulin-BindingProteinGenefromCitrusGrandisvar.ShatinyuHortQiongLuo,HongniTan,ShiliHuang,HuayingLiu,
2、XinminQin*CollegeofLifeScience,GuangxiNormalUniversity,GuilinGuangxithththReceived:May6,2017;accepted:May20,2017;published:May27,2017AbstractInthispaper,thetranscriptomeoftheself-pollinatedstyleandcross-pollinatedstyleofCitrusgrandisvar.ShatinyuHortweresequencedbyhigh-throughputsequencingte
3、chnology.Acalmo-dulin-bindingproteingenesequenceofCitrusgrandisvar.ShatinyuHortwasobtainedthoughdif-ferentialanalysismethod,andsomecharactersofthecalmodulin-bindingproteinwereanalyzedandpredictedbybioinformaticsmethod,includingthecompositionofaminoacidsequence,phy-sicochemicalparameters,hyd
4、rophobicity,transmembranedomain,secondarystructureandfunc-tionofproteinetc.Theresultsshowedthatthecalmodulin-bindingproteingenewas1616bpinlengthwithanopenreadingframe(ORF)of1368bp,encoding455aminoacidswithdeducedmo-lecularweightof50.11KDa,andtheoreticalpIvalueof5.33,andcontainedacalmodulin-
5、bindingproteinsdomain.Bioinformaticsanalysisshowedthatthecalmodulin-bindingproteinwasahy-drophobicandunstableprotein.Thehomologyanalysisofaminoacidsequenceindicatedthatthecalmodulin-bindingproteinsharedhighhomologywithcalmodulin-bindingproteinofCitruscle-mentina(98%).Thisworkprovidesasystem
6、icsequenceanalysisofthecalmodulin-bindingpro-teinofCitrusgrandisvar.ShatinyuHort.Itwillprovidetheusefulreferenceforfurtherinvestiga-tionofitsstructureandfunction．KeywordsCitrusGrandisvar.ShatinyuHort,Calmodulin-BindingProteinGene,SequenceAnalysis沙田柚钙调素结合蛋白(CaMBP)基因的鉴定与序列分析*罗琼，谭鸿妮，黄仕莉，刘华英，秦新
7、民广西师范大学生命科学学院，广西桂林*通讯作者。文章引用:罗琼,谭鸿妮,黄仕莉,刘华英,秦新民.沙田柚钙调素结合蛋白(CaMBP)基因的鉴定与序列分析[J].自然科学,2017,5(2):203-210.https://doi.org/10.12677/ojns.2017.52027罗琼等收稿日期：2017年5月6日；录用日期：2017年5月20日；发布日期：2017年5月27日摘要利用高通量测序对沙田柚自交和异交花柱进行转录组测序，通过差异分析得到了沙田柚钙调素结合蛋白(CaMBP)基因序列。采用生物
