粉防己碱预处理减轻大鼠肝脏缺血再灌注损伤的机制研究

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1、粉防己碱预处理减轻大鼠肝脏缺血再灌注损伤的机制研究作者：刘忠民　熊进文　林智敏　徐振萍　沈文律　　【摘要】目的研究粉防己碱(tetrandrine,TET)预处理对大鼠肝缺血再灌注损伤的预防作用及其机制。方法大鼠肝脏左中叶缺血50min，再灌注24h后处死(I/R组)，TET+I/R组缺血前30min腹腔注射TET(50mg/kg)，余同I/R组。大鼠处死前采血检测丙氨酸氨基转移酶(ALT),天门冬氨酸氨基转移酶(AST),乳酸脱氢酶(LDH),取缺血肝组织进行超氧化物歧化酶(SOD),一氧化氮(NO)，丙二醛(MDA)，湿重干重(W/D)比检测和组织学检查。结果I/R组血清AL

2、T,AST和LDH升高，组织MDA水平,W/D比值也明显升高，SOD活性和NO含量下降。在TET+I/R组，血清ALT,AST,LDH，以及组织W/D比值较I/R组降低，SOD活性和NO含量升高。此外，TET+I/R组大鼠血清ALT,AST,组织MDA和W/D比值较假手术组升高，SOD活性和NO含量降低.组织学检查显示TET+I/R组肝损害减轻。结论TET能减轻但不能防止肝I/R损伤的发生，减少脂质过氧化物产生是TET抗I/R损伤的原因之一。　　【关键词】粉防己碱；缺血再灌注；大鼠　　　　【Abstract】ObjectiveTheeffectoftetrandrine(TE

3、T)pretreatmentofWistarratssubjectedtowarmhepaticischemia/reperfusion(I/R)wasinvestigated.MethedsAfter50minutesofischemiaintheleftandmedianlobesofthe8liverand24hoursofreperfusion(I/Rgroup),theratswerekilled.TheTET+I/RgroupratswerepretreatedwithTET(50mg/kgbodyweightIP)30minutespriortotheonsetof

4、ischemia.Bloodsamplesweretakenformeasurementofalanineaminotransferase(ALT),aspartateaminotransferase(AST),andlactatedehydrogenase(LDH).Tissuewastakenfromtheischemiclobesformeasurementofsuperoxidedismutase(SOD),malonyldialdehyde(MDA),andmyeloperoxidase(MPO);determinationofthewet/dryweight(W/D)

5、ratio;andhistologicstudies.ResultsTheresultsshowedthatALT,AST,andLDHlevelsinserumwereincreasedintheI/Rgroup;tissueMDAgeneration,MPOactivity,andtheW/Dratiowerealsoincreased,accompaniedbydecreasedSODactivity.TheserumALT,AST,andLDHlevels,aswellasthetissueMPOlevelandW/Dratio,werelowerintheTET+I/R

6、groupthanintheI/Rgroup;andtheSODlevelwashigherintheTET+IRgroupthanintheI/Rgroup.Moreover,theserumALTandAST,tissueMDA,andW/DratiointheTET+I/Rgroupwerehigher,andtheSODwaslowerthanintheshamgroup.ThehistologicexaminationshowedprotectionagainstliverdamageintheTET+I/Rgroup.ConclusionTheresultsdemon

7、stratedthatpretreatmentwithTETcouldsomewhatprotecttheliveragainstI/Rinjurybut8doesnotpreventit.Thesimultaneousdecreaseofbothlipidperoxidegenerationandpolymorphonuclearneutrophilinfiltrationintheischemiclivermayexplaintheacquisitionoftolerance