抗纤方对肝纤维化大鼠肝组织金属蛋白酶及其抑制因子表达的影响

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1、湖北中医学院硕士学位论文抗纤方对肝纤维化大鼠肝组织金属蛋白酶及其抑制因子表达的影响姓名：张爱忠申请学位级别：硕士专业：中医临床基础指导教师：伍定邦2003.6.1．塑些生鉴兰壁矍主!些丝塞——ABSTl淑CTObjective：ToreproducehepaticfibrosismodelofratsandobservetheeffectofKangXianRuanGanChongJi(KXRGCJ)intreatmentofdifferentgropeofthoserats，especiallytoobservetheeffectonmat

2、rixmetalloproteinase一1(醚挺P—t)、tissueinhibitorsofmetat—loproteinase一1(TI酣P—I)mRNAexpression，Soastostudythemechan—iSillofKXrGCJNethods：Experimentoftreatinghepaticfibrosis：Fromthebeginningtomakemodels，90wistarratswererandomlydividedinto6groups：normalcontrolgrope(10)、ultrastruc

3、turegrope(16)、colchicinesgrope(16)、andECM(KXrGCJ)gropes(16respectively)。Exceptthenormalgrope，alloftheothers(themodelgropes)wereinducedtohepaticfibrosisbyCOl·andadministeredrespectivelywithwateratthedoseof2ml／1009／d(theultrastructuregrope)、colchicinesatthedoseoflmg／lOOg／d(th

4、ecolchicinesgrope)、ECMatthedosesof0．69、1．29、2．49／lOOg／d(thelow、middle、highdoseECMgrope)。Attheendoftheeii出thweek，alloftheremnantratswerekilled，Somepartsof1iverweretakenwhichwouldbeusedintheexperimenteResult：(1)HP一1expression．．Comparewiththeultrastructuregrope，the1eveloftheno

5、rmalgropeiSmodestlylower，butthedifferenceiSnotmarked(P>O．05)．thelevelsofthegropestreatedwithmedicinearemarkedlyhigher(PO．05)．thelevelSofthemiddleandhighdose袋瀑gro

6、pesaremarkedlyincreased(P<&05)：Comparewiththemiddledosegrope，thelevelofthelowdoseECMiSmarkedlylower(PO．05)．(DTIMP一1expression：themodelgropesaresignificantlyhigherthanthatofthenormalgrope(P(0。0

7、1)；Comparewiththeuttrastructuregrope，thelevelsofthegropestreatedwithmedicinearemarkedlylower(P>O．05)：Comparewiththecolchicinesgrope，thelevelofthelowdoseECMgropeismodestlylower。butthediffer-enceiSnotmarked(P>O．05)，thelevelSofthemiddleandhighdoseECMgropesaremarkedlydecreased(

8、K袋05)tComparewiththemiddle-2-潮嚣巾医学院磺士毕监论义dosegrope，thelevelofthelowdoseECMismarked