重症肌无力胸腺差异蛋白质组学研究

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1、郑州硕士学论文题目：作者姓名：学科门类：专业名称：导师姓名、职称：授予单位代码!Q笪里学号或申请号——密级垒五大一学位论文重症肌无力胸腺差异蛋白质组学研究侯智亮基础医学免疫学’杜英教授张清勇教授2007年5月18日郑州大学2007届硕士研究生论文中文摘要的生物学功能，进一步探讨与MG发生、发展及预后的关系以及寻找崛生物治疗的靶蛋白打下了基础。关键词：重症肌无力蛋白质组学双向电泳基质辅助激光解析电离飞行时间质谱¨郑州大学2007届硕士研究生论文英文摘要Studyoftheproteomicsbetweenthed

2、onorsandthehyperplasticthymustissuefrompatientswithmyastheniagravisApplicantforMasterDegreeHouZhiliangTutorsDuYingZhangQingyongDepartmentofMicrobiologyandImmunology,SchoolofBasicMedicalSciences，ZhengzhouUniversity,Zhengzhou450001DepartmentofThoracicSurgery，t

3、hesecondAffiliatedHospital，ZhengzhouUniversityZhcngzhou450014AbstractObjectives：Tocomparetheresultsofproteomicsbetweenthedonorsandthehyperplasticthymustissuefrompatientswithmyastheniagravis．Methods：Theproteinsisolatedfromthehyperplasticthymustissuefrom21pati

4、entswithmyastheniagravis(MG)and11donorswereseparatedbytechniquesof2-DE(Two—dimensionalgelelectrophoresis)．Theproteinswerethenstainedwithcolloidalcoomassiebluetoproduceahigh—resolutionmapoftileproteome．Selectedproteinsfromthismapwerein—geldigestedwithtrypsina

5、ndtheresultingtrypticpeptideswereanalyzedbymatrixassistedlaserdesorptionionizationtimeofflightmassspectrometry(MALDI_TOF—MS)．ProteinidentificationwasfinishedbysearchingthePMFdatainNCBInrproteindatabankusingMascotprogramatb±!Q；』Z!!型：堡垒l!i』§￡i￡n￡￡：￡Q型．Results：

6、10abnormalproteinspotswereobtained．8ofwitchwereidentificated，2wereunidentifled．．Therewere4spotssignificantlyincreased，whicharetubulin，beta、ChainA，ThioredoxinPeroxidaseB、III郑州大学2007届硕士研究生论文英文摘要EI卜60protease、ChainA．CrystalStructureOfTheGaModuleComplexedWithHum

7、anSerumAlbumin；4spotssignificantlydecreased，whicharebiliverdinreductaseB、Keratin，typeIcytoskeletal19、ChainB，T-To-T(High)QuaternaryTransitionsInHumanHemoglobin：Deshisl46betaDeoxyLow—Salt、a1IoalbuminVeneziaConclusions：Theseproteinsfromhyperplasticthymustissuef

8、rompatientswithmyastheniagravis(MG)weredifferentofthedonors．．Itwillbehelpfultofurtherresearchof粥andfindnewmolecularmarkersforearlydiagnosisandtreatmentofMG．Keywords：myastheniagravis，comparativep