TOLL样受体在尖锐湿疣中表达的研究

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1、上海交通大学硕士学位论文TOLL样受体在尖锐湿疣中表达的研究姓名：徐佩红申请学位级别：硕士专业：皮肤性病学指导教师：郑捷;袁定芬20070401TOLL样受体在尖锐湿疣中表达的研究细胞中TLR2、TLR3的表达水平较健康对照组显著减少，两组间差异有显著性差异，而TLR9在两组中均未见表达。提示尖锐湿疣患者局部天然免疫功能低下使尖锐湿疣病毒发生免疫逃逸可能在尖锐湿疣发病中起作用。结论：在尖锐湿疣发生发展过程中，HPV感染局部的天然免疫缺陷，使尖锐湿疣病毒发生免疫逃逸，可能在尖锐湿疣发病中起主要作用。关键词：尖锐湿疣，HPV，Toll样受体，免疫VTOLL样受体在尖锐湿疣中表达的研究

2、THESTUDYONTHEEXPRESSIONSOFTOLL-LIKERECEPTORSINTHECONDYLOMATAACUMINATAABSTRACTCondylomataacuminataisapapilliformhyperplasiaofepidermiscausedbyhumanpapillomavirus.Along-timeclinicalobservationandstudiesrevealthathostimmuneconditionhasanintimaterelationshiptothiscommondisease,whichmayberesponsib

3、lefortheinfectionofHPV,developmentandrecurrenceofthedisease.Tolllikereceptors(TLRs)areknownaspatternrecognitionreceptors,whichcanrecognizeavarietynumberofmicrobesincludingvirus.However,itisstillunclearthemechanismofinnateimmunityhowinfectiontriggerscondylomataacuminata.Therefore,inordertoinve

4、stigatethecontributionofinnateimmunityinthedevelopmentofcondylomataacuminata,westudiedtheexpressionofTolllikereceptorsinthepatients1.TheexpressionsofTLR2、TLR3andTLR9mRNAintheperipheralbloodmononuclearcells(PBMCs)frompatientsandhealthyVITOLL样受体在尖锐湿疣中表达的研究controlsweredetectedbyreal-timequantita

5、tivePCR.WefoundthatthelevelsofTLR2,TLR3andTLR9mRNAhadnosignificantlydifferencesbetweenpatientsandhealthycontrols.Itissuggestedthatsystemicimmunitymaynotplayaroleinthepathogenesisofcondylomataacuminate.2.TheexpressionsofTLR2、TLR3andTLR9inthekeratinocytesfrompatientsandhealthycontrolsweredetect

6、edbyimmunohistochemicalstaining.WefoundthatthelevelsofTLR2,TLR3inthepatientshadsignificantlydownregulated.TLR9wasnotfoundinbothgroups.Theresultsindicatedthattheinhibitionofinnateimmunityintheinfectedregionsmayplayamainroleinthepathogenesisofcondylomataacuminate.Inaword,ourstudyfirstlyconfirme

7、dthattheinhibitionofinnateimmunityintheinfectedregionsmayplayamainroleinthepathogenesisofcondylomataacuminate.ThepathogenesisofcondylomataacuminatemayduetotheescapeofHPVfrominnateimmunity.KEYWORDS:condylomataacuminate,HPV,Tolllikereceptors,im