长链非编码rna分子uca1介导胃癌多药耐药的机制研究

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1、第四军医大学硕士学位论文分类号R735.2UDC616-006密级公开长链非编码RNA分子UCA1介导胃癌多药耐药的机制研究张哲培养类别全日制学位类型学术学位一级学科(专业类)临床医学二级学科(专业)内科学（消化系病）研究方向胃癌多药耐药指导教师樊代明教授（主任医师）培养单位西京消化病医院二O一五年五月第四军医大学硕士学位论文第四军医大学硕士学位论文目录缩略语表······································································

2、·························1中文摘要·······························································································5英文摘要·······························································································8前言························

3、······································································12文献回顾······························································································13正文··············································································

4、················32第一部分长链非编码RNA芯片筛选及UCA1的表达验证·····························321材料····························································································321.1细胞系··········································································

5、········321.2主要试剂···············································································331.3主要仪器···············································································332方法·······························································

6、·····························332.1细胞培养···············································································332.2Trizol法提取细胞总RNA··························································342.3qRT-PCR验证lncRNA的表达··································

7、·················343结果····························································································364讨论····························································································41第二部分LncRNAUCA1对胃癌多药耐药的调节作用·············

8、·····················431材料····························································································431.1细胞系··················································································431.2主要试剂···················