角质形成细胞抗原提呈功能研究

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1、分类号R758.69UDC616.5密级公开角质形成细胞抗原提呈功能研究郝军峰培养类别全日制学位类型专业学位一级学科(专业类)临床医学二级学科(专业)皮肤病与性病学研究方向角质形成细胞指导教师王刚教授（主任医师）培养单位第四军医大学西京皮肤医院二O一五年五月第四军医大学硕士学位论文第四军医大学硕士学位论文目录缩略语表·······························································································1中文摘要···········

2、····················································································3英文摘要·······························································································5前言···················································································

3、··················8文献回顾······························································································10正文····································································································20角质形成细胞抗原提呈功能研究·································

4、····························201材料····························································································201.1主要实验仪器·········································································201.2主要实验试剂··················································

5、·······················211.3主要试剂配方·········································································231.4实验中所用的细胞系································································271.5MHC分型检测·······································································27

6、2方法····························································································272.1HaCaT细胞表达抗原提呈相关分子MHC-I、MHC-II、ICAM-1的研究.272.2HaCaT细胞MHC分型检测·······················································302.3MHC结合抗原肽的免疫共沉淀（Co-IP）实验····························

7、···312.4MHC结合抗原肽的质谱鉴定·····················································352.5HaCaT细胞活化T细胞的研究···················································352.6统计方法···············································································373结果································

8、······························································374讨论···········································