胸腺内注射供体bmscs对同种异体腹部皮瓣移植排斥反应影响的研究

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1、分类号R392.4UDC616-097密级公开胸腺内注射供体BMSCs对同种异体腹部皮瓣移植排斥反应影响的研究张栋梁培养类别全日制学位类型学术学位一级学科(专业类)临床医学二级学科(专业)外科学（整形外科）研究方向异体复合组织移植指导教师郭树忠教授（主任医师）培养单位西京医院整形外科二O一五年五月第四军医大学硕士学位论文目录缩略语表··························································································

2、·····1中文摘要·······························································································2英文摘要·······························································································5前言·················································

3、··············································8文献回顾······························································································10正文······························································································24第一部分BN大鼠BMSC

4、s的分离培养、纯化与鉴定·································241材料····························································································241.1实验动物················································································241.2实验试剂·················

5、·······························································251.3实验器材················································································262方法····························································································262.1BN大

6、鼠BMSCs的分离培养与纯化···············································262.2BN大鼠BMSCs的鉴定······························································273结果····························································································283.1培养过程中的形态学观察····

7、·······················································283.2表型鉴定结果··········································································293.3分化能力鉴定结果····································································304讨论·······························

8、·····························································31第二部分胸腺内注射供体BMSCs对同种异体腹部皮瓣移植排斥的影响及机制初探·341材料····························································································341