mir-182对瘦素诱导的卵巢癌细胞生长的影响及调控机制

《mir-182对瘦素诱导的卵巢癌细胞生长的影响及调控机制》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、分类号R71UDC618密级公开miR-182对瘦素诱导的卵巢癌细胞生长的影响及调控机制姜霞培养类别非全日制学位类型学术学位一级学科(专业类)临床医学二级学科(专业)妇产科学研究方向妇科肿瘤指导教师于月成副教授（副主任医师）培养单位西京医院妇产科二O一五年十月第四军医大学硕士学位论文目录缩略语表·········································································································1中文摘要························

2、·················································································2英文摘要·········································································································5前言························································································

3、·················8文献回顾·······································································································10正文·······································································································24第一部分miR-182和瘦素在卵巢癌患者组织中的表达及其与卵巢癌发展的相关性研究··241资料·

4、···································································································241.1研究对象························································································241.2纳入排除标准···········································································

5、·······241.3观察指标························································································251.4实验试剂························································································251.5实验仪器········································································

6、················262方法····································································································262.1RT-PCR检测两组患者组织中瘦素mRNA表达········································262.2蛋白质印迹法检测两组患者组织中瘦素水平············································312.3RT-qPCR检测两组患者组织中miR-

7、182表达···········································282.4酶联免疫吸附法检查卵巢癌患者血清和尿液中的瘦素水平·························332.5统计学方法·····················································································343结果···············································································

8、·····················343.1