过表达ndrg2基因促进人髓核细胞衰老的体外实验研究

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1、第四军医大学分类号R68UDC617.3TheFourthMilitaryMedicalUniversity密级公开过表达NDRG2基因促进人髓核细胞衰老的体外实验研究刘永明培养类别全日制学位类型学术学位一级学科(专业类)临床医学二级学科(专业)外科学（骨外）研究方向脊柱脊髓损伤基础与临床指导教师钱济先教授（主任医师）培养单位唐都医院骨科二O一五年五月第四军医大学硕士学位论文目录缩略语表················································································

2、··············1中文摘要······························································································3英文摘要······························································································6前言····························································

3、··································9文献回顾·····························································································11正文·····························································································22第一部分过表达NDRG2髓核细胞系的构建及髓核细胞中过表达P53基因对NDRG2表达的影

4、响······························································221材料···························································································222方法···························································································243结果···············

5、············································································274讨论···························································································30第二部分过表达NDRG2对髓核细胞生物学功能的影响····························361材料···········································

6、················································362方法···························································································373结果···························································································394讨论······························

7、·····························································42小结·····························································································46参考文献·····························································································48个人简历和研究成果·······

8、·······································································54致谢