显齿蛇葡萄中黄酮类化合物代谢积累及相关性研究

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1、学校代码：10663学号：4201210000533贵州师范大学硕士学位论文显齿蛇葡萄中黄酮类化合物代谢积累及相关性研究StudyontheMetabolicAccumulationandCorrelationofFlavonoidAglyconesinAmpelopsisgrossedentata专业名称：生物化学与分子生物学专业代码：071010研究方向：代谢组学申请人姓名:王家胜导师姓名（职称）：余正文（教授）二〇一五年五月四日摘要本研究以HPLC等仪器分析手段测定显齿蛇葡萄Ampelopsisgrossedenta

2、ta(Hand.-Mazz.)W.T.Wang中二氢杨梅素及其生源相关黄酮苷元的含量，并借助SPSS软件分析相关性，明确二氢杨梅素代谢积累的关键前体及竞争性底物。1）建立了HPLC分析检测显齿蛇葡萄叶中二氢杨梅素的方法，2回归方程为：Y=28327X-101.2(R=0.996)，定量限为35.74ng，在0.5~10.0μg范围内线性关系良好。2）建立了显齿蛇葡萄中二氢杨梅素和杨梅素HPLC同时分析的HPLC-UV法，该方法稳定性、重现性和精密度均较好。3）利用PTLC-HPLC法建立了二氢杨梅素、杨梅素、槲皮素等11种

3、黄酮苷元同时检测的方法。4）建立了显齿蛇葡萄中总黄酮含量测定的紫外分光光度法，为显齿蛇葡萄资源评价提供了技术参考。研究结果为进一步探求二氢杨梅素的代谢积累的分子机制提供参考。关键词显齿蛇葡萄；黄酮苷元；代谢产物；相关性中图分类号：Q946.889/Q949.95IAbstractsAnalyticalinstrumentsashigh-performanceliquidchromatography(HPLC)wereadoptedtodetectthecontentofdihydromyricetinandrelatedf

4、lavonolsinAmpelopsisgrossedentata(Hand.-Mazz.)W.T.Wang,andtheSPSS22.0softwarewasusedtoanalyzethecorrelationandilluminatethekeyprecursorsandcompetitivesubstratesinvolvedinthebiosyntheticpathwayofdihydromyricetin.1)ThemethodtodetectthecontentofdihydromyricetininA.gr

5、ossedentatawasestablished,andthelinearregressionequationcould2bestatedasfollows:Y=28327X-101.2(R=0.996),withalimitofquantity(LOQ)at35.74ng,whichshowedagoodlinearrelationshipintherangeof0.5μg～10.0μg.2)AwayonsimultaneousanalysisofdihydromyricetinandmyricetinbytheHPL

6、C-UVmethodhasbeenestablished,whichhasgoodstability,reproducibilityandprecision.3)Toanalyzetheelevenflavonolsincludingdihydromyricetin,myricetinandquercetinsimultaneously,PTLC-HPLCmethodhasbeenestablished.4)TheapplicationofsimpleUV-Visspectrophotometrytechniquewasf

7、orthedetectionofthetotalflavonoidsinA.grossedentata,whichcouldprovideaneffectivetechnicalreferencefortheappraisalofA.grossedentata.TheresearchwillprovidereferenceforfurtherstudyonthemolecularmechanismofthemetabolicaccumulationofdihydromyricetininA.grossedentata.Ke

8、ywords：Ampelopsisgrossedentata;Flavonoidaglycones;Metabolites;CorrelationII目录摘要.........................................................................