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时间:2019-06-19
《显齿蛇葡萄叶对食品防腐作用的研究_刘胜贵》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、ScienceandTechnologyofFoodIndustry研究与探讨显齿蛇葡萄叶对食品防腐作用的研究刘胜贵,张静,王频艺,付明(怀化学院生命科学系,民族药作植物资源研究与利用湖南省重点实验室,湖南怀化418008)摘要:为了研究显齿蛇葡萄对食品的防腐效果,分别采摘3、7、11月份的显齿蛇葡萄叶作为实验材料,以大肠杆菌、金黄色葡萄球菌、苏云金芽胞杆菌、藤黄八叠球菌和枯草芽胞杆菌五种微生物为实验菌株,用药敏纸片扩散法测定不同生长时期的显齿蛇葡萄叶抑菌圈直径,通过二倍稀释法测定MBC值,以平板菌落计数反映添加有不同提取物浓度的食品中微生物的生长状况。结果表
2、明7月份采摘的显齿蛇葡萄提取物对金黄色葡萄球菌抑菌作用最强,抑菌圈直径达到27.0mm,MBC为5%;当提取物浓度为4MBC时,肉汤食品抑菌时间延长达140h。显齿蛇葡萄有广谱抑菌作用,并且对猪肉、牛奶、西瓜和豆浆都有防腐作用。关键词:显齿蛇葡萄,水提取物,药敏纸片扩散法,二倍稀释法,平板菌落计数法ApplicationonfoodpreservationofAmpelopsisgrossedentataleavesLIUSheng-gui,ZHANGJing,WANGPin-yi,FUMing(TheKeyLaboratoryofResearchandUtil
3、izationofEthnomedicinalPlantResourcesofHUnanProvince,DepartmentofLifeSciences,HuaihuaUniversity,Huaihua418008,China)Abstract:ToresearchtheapplicationonfoodpreservationofextractofAmpelopsisgrossedntata,theleavesofA.grossedentatathatwerecollectedinMarch,JulyandNovemberweretakenasexperi
4、mentalmaterials,respectively.Escheridhiacoli,Staphylococcusaureus,Bacillusthuringiensis,SarcinaluteaandBacillussubtilisweretakenasexperimentalstrains.AfterthedifferentbacteriaweredealedwithleavesextractsofA.grmsedntara,theinhibitoryzonediameterswereevaluatedbyusingadisk-diffusionandt
5、heminimumbactericidalconcentrations(MBC)weredetectedbydoubledilutionmethodduringtheprocessofbacterialgrowth.Microorganismsinthefoodsolution,whichwererelatedtodifferentconcentrationsoftheextracts,werequantifiedbycolonycountingmethod.TheresultsshowedtheextractsfromtheleavesofA.grossede
6、ntatawerecollectedinJulyhadthestrongestantibacterialeffectonS.aureus,theinhibitoryzonediameterreached27.0mmandMBCwasonly5%.Bacteriostatictimeforbrothfoodcouldreach140hwhentheconcentrationoftheextractswas4MBC.ThisindicatedtheextractsfromtheleavesofA.grossedentatahadbroadantimicrobials
7、pectrumandgoodpreservativeeffectonpork,milk,watermelonandsoymilk.Keywords:Ampelopsisgrossedentata;waterextracts;drugsensitiveslipsmethod;doubledilutionmethod;plateculturecountmethod中图分类号:TS201.3文献标识码:A文章编号:1002-0306(2014)08-0118-04doi:10.13386/j.issn1002-0306.2014.08.017显齿蛇葡萄(Ampelop
8、sisgrosseden
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