口腔乳酸杆菌的分离鉴定及其益生功能的研究

《口腔乳酸杆菌的分离鉴定及其益生功能的研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、分类号R1学号152122ＵＤＣ614密级公开军事科学院博（硕）士学位论文口腔乳酸杆菌的分离鉴定及其益生功能的研究Isolationandidentificationoforallactobacillusandthestudyofitsprobioticfunction作者姓名黄思妺学科专业军事预防医学指导教师黄留玉研究员答辩主席黄培堂研究员学位授予单位军事科学院论文提交日期2018年5月18日军事科学院博（硕）士学位论文目录摘要................................................................................

2、.....................................................IAbstract...............................................................................................................................IV前言........................................................................................................

3、............................1第一章分离筛选优质乳酸杆菌..........................................................................................61实验材料...............................................................................................................81.1菌株..........................................

4、..................................................................81.2试剂耗材.....................................................................................................81.3仪器设备....................................................................................................92实验方法........

5、.......................................................................................................92.1人群选择....................................................................................................92.2唾液样本的采集...........................................................................

6、...........102.3乳酸杆菌的分离和DNA提取...............................................................102.4对细菌基因组16s区域进行PCR扩增.................................................112.5测序结果比对分析..................................................................................112.6API细菌鉴定系统进行菌种鉴定..................

7、.......................................122.7菌种的复苏、培养和保存......................................................................122.8pH测定....................................................................................