高分辨率溶解曲线（hrm）技术在高血压患者mthfr基因多态性筛查中的应用

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1、授予单位代码10089学号或申请号13360HebeiMedicalUniversity硕士学位论文科学学位高分辨率溶解曲线（HRM）技术在高血压患者MTHFR基因多态性筛查中的应用作者姓名：李玲导师：李拥军教授专业：心内科二级学院：河北医科大学第二医院2015年08月河北医科大学学位论文使用授权及知识产权归属承诺本学位论文在导师，由本人独立完成。（或指导小组）的指导下本学位论文研究所获得的研究成果，其知识产权归河北医科大学所有。河北医科大学有权对本学位论文进行交流、公开和使用？凡发表一与学位

2、论文主要内容相关的论文，第署名为单位河北医科大学，试验材稱、原始数据、申报的专利等知识产权均归河北医科大学所有。。否则，承担相应法律责任研巧生签名导师三二级学院领琴盖韋’Ｉ年吊月日、＇Ｉｆ河北医科大学研究生学位论文独创性声明本论文是在导师指导下进行的研究工作及取得的研究成果，除了文中特别加Ｗ标注和致谢等内容外，文中不包含其他人曰经发表或撰写的研究成果，指导教师对此进行了审定。本论文由本人独立撰写。，文责自负研究生签名：导师签章＾＾兩０＾曼年１月曰目录中

3、文摘要··············································································1英文摘要··············································································4英文缩写··············································································7研究论文高分辨率溶解曲

4、线（HRM）技术在高血压患者MTHFR基因多态性筛查中的应用前言··············································································8材料与方法·····································································11结果······································································

5、········17附图··············································································18附表··············································································20讨论··············································································22结论·

6、·············································································26参考文献········································································26综述高分辨率熔解曲线分析技术及其应用进展····························30致谢··················································

7、··································39个人简历··············································································40中文摘要高分辨率溶解曲线（HRM）技术在高血压患者MTHFR基因多态性筛查中的应用摘要目的：H型高血压为伴有高同型半胱氨酸血症（homocysteine，Hcy）的原发性高血压，近年来越来越受到人们的重视，它以血Hcy水平增高为特点（Hcy≥10μmol/L），是动脉粥样硬化的

8、独立危险因素，与外周血管病变、脑血管病变、高血压、高血压性心脏病、冠状动脉阻塞及血栓形成的发生密切相关。人类亚甲基四氢叶酸还原酶（methylenetetrahydrofolatereductase，MTHFR）是叶酸代谢中的关键酶之一，可将还原型辅酶I（NADPH）相关的5,10-亚甲基四氢叶酸还原为5-甲基四氢叶酸，这一代谢途径的正常运转在维持血浆中同型半胱氨酸正常水