食管鳞癌淋巴结转移相关的外泌体microRNAs表达谱研究

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1、分类号：R181.3学校代码：10392学科专业代码：100401学号：2130305623硕士学位论文食管鳞癌淋巴结转移相关的外泌体microRNAs表达谱研究ThepreliminaryanalysisofexosomalmicroRNAsexpressionprofileinlymphnodemetastasisofesophagealsquamouscellcarcinoma学位类型：医学硕士所在学院：公共卫生学院申请人姓名：杨晓鹭学科、专业：流行病与卫生统计学导师：胡志坚教授研究起止日期：2014年

2、09月至2016年03月答辩委员会主席：郑奎城教授答辩日期：2016年05月27日二○一六年五月目录中文摘要···········································································································01ABSTRACT······················································································

3、·····················03前言···················································································································05第一部分食管鳞癌淋巴结转移患者血浆外泌体中microRNAs表达谱的分析研究·············································································

4、·············································07一、材料与方法···························································································071.研究对象······························································································072.标本收集与处

5、理···················································································093.样本分组情况·······················································································094.主要仪器设备及试剂·······························································

6、·············095.实验方法······························································································09二、实验结果······························································································131.研究对象的基本特征···························

7、·················································132.外泌体的形态学观察············································································133.WesternBlot定性检测外泌体分子标志·············································144.RNA质检结果·····································

8、···················································145.差异性表达基因的筛查结果·································································14三、讨论·······························································