表达外源性中分子量神经细丝及核转位对细胞凋亡的影响

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1、分类____________学号M201175038学校代码10487密级___________硕士学位论文表达外源性中分子量神经细丝及核转位对细胞凋亡的影响学位申请人：张敏学科专业：人体解剖与组织胚胎学指导教师：李宏莲副教授答辩日期：2013年12月万方数据MASTER’SDISSERTATIONEffectofexpressionalexogenousmiddlemolecularweightneurofilamentanditsnucleartranslocationoncellapoptosisCandidate:ZhangMinSupervisor:Ass

2、ociatedProf.LiHLMajor:HistologyandEmbryologyTongjiMedicalCollegeHuazhongUniversityofScienceandTechnologyWuhan,Hubei430030,P.R.ChinaDecember2013万方数据独创性声明本人声明所呈交的学位论文是我个人在导师指导下进行的研究工作及取得的研究成果。尽我所知，除文中已经标明引用的内容外，本论文不包含任何其他个人或集体已经发表或撰写过的研究成果。对本文的研究做出贡献的个人和集体，均已在文中以明确方式标明。本人完全意识到，本声明的法律结果由本

3、人承担。学位论文作者签名：日期：年月日学位论文版权使用授权书本学位论文作者完全了解学校有关保留、使用学位论文的规定，即：学校有权保留并向国家有关部门或机构送交论文的复印件和电子版，允许论文被查阅和借阅。本人授权华中科技大学可以将本学位论文的全部或部分内容编入有关数据库进行检索，可以采用影印、缩印或扫描等复制手段保存和汇编本学位论文。保密□，在___年解密后适用本授权书。本论文属于不保密□（请在以上方框内打“√”）学位论文作者签名：指导教师签名：日期：年月日日期：年月万方数据目录中文摘要·········································

4、·······································1英文摘要················································································2正文部分表达外源性中分子量神经细丝及核转位对细胞凋亡的影响前言················································································3材料与方法············································

5、·························4结果··············································································11讨论··············································································16参考文献········································································18综述················

6、····································································20参考文献··············································································36致谢····················································································46万方数据华中科技大学硕士学位论文表达外源性中分子量神经细丝及核转位对细胞凋亡的影响硕士研

7、究生：张敏导师：李宏莲教授摘要：早老性痴呆（AlzheimerDisease，AD）和其它多种神经退行性疾病均有神经原纤维缠结（NFT）存在于大脑，NFT由过度磷酸化的微管相关蛋白tau、神经细丝（neurofilament，NF）等组成。很多研究已证实过表达tau有抗凋亡的作用，且胞核内的去磷酸化tau对DNA有保护作用，但是关于神经细丝对细胞生存的影响还不清楚。本研究的目的是探讨外源性NFM及其细胞内的定位对细胞凋亡的影响。将中分子量的神经细丝（NFM）和人全长的tau（tau441）的质粒（EGFP-C1-NFM、EGFP-C1-tau和HA-C1-NFM