海带多糖在2型糖尿病小鼠中的降糖作用和机制

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1、海带多糖在2型糖尿病小鼠中的降糖作用和机制摘要目的：观察2型糖尿病小鼠在海带多糖干预下，空腹血糖、血胰岛素、血胰淀素、胰岛素受体以及肝糖原合成酶的变化，探讨海带多糖在实验性2型糖尿病小鼠中的降血糖作用和可能的机制。方法：健康雄性昆明小鼠60只，高脂饲料喂养并腹腔注射四氧嘧啶制备2型糖尿病动物模型，昆布多糖灌胃干预治疗。自动血糖仪测小鼠空腹血糖(FBG)，免疫电化学发光法检测血清胰岛素(Insulin)水平，酶联免疫吸附法检血清胰淀素(Amylin)水平，肝糖原合成酶(Glycogensynthase)和脑干组织胰淀素受体(Amylinreceptor)水平。结果：经海带

2、多糖干预治疗后，高．中．低剂量组动物FBG水平较模型组显著降低(尸0．05)；上述指标在治疗各剂量组之间均无明显差异(胗O．05)。结论：海带多糖在2型糖尿病早期，能促进胰岛细胞的分泌功能，提高血清胰岛素和胰淀素水平，发挥降糖作用。硕士研究生：龙少华指导教师：郭云良【关键词】海带多糖；2型糖尿病；四氧嘧啶；胰岛素；胰淀素；小鼠lIllllllllllllllllllllllllllllllllllllllllH

3、Y2338732THEHYPOGLYCEMICEFFECTANDMECHANISMOFLAMINARIAJAPONICAPOLYSACCHARIDEINTYPE2DIABETESMELLITUSMODELMICEABSTRACTObjective：Toobservedegreeschangeoffastingblood—glucose(FBG)，insulin，amylin，amylinreceptorandglycogensynthaseafteractedbyLaminariaJaponicapolysaccharide(LJPS)intype2diabetesme

4、llitus(DM)modelmice．ToinvestigatethehypoglycemiceffectanduseofLJPSintype2diabetesmellitus(DM)modelmice．Methods：Type2DMmodelswereestablishedin60healthymalemicethroughfeedingfat-richforageandinjectingalloxan．LJPSwasaddedtonormalsalineandadministeredoralytotreatthemice．Fastingbloodglucose(F

5、BG)levelsweredetectedusingallautomaticbloodglucosedevice．ElectrochemiluminescenceimmunoaSsay(ECLIA)wasappliedtodetermineseruminsulinlevel．Enzymelinkedimmunosorbentassay(ELISA)determinedamylinlevelinserum，glycogensynthaselevelinliver,andamylinreceptorlevelinthebrainstem。Results：AfterLJPSt

6、reatment，FBGinthelow,mediumandhighdosagegroupsdecreasedsignificantlyincomparison晰tllthemodelgroup俨<0．05)，andthelevelofinsulinandamylininserumincreasedsignificantlyincomparisonwitllthemodelgroupfP<0．05)．Therewerenosignificantdifferencesinliverglycogensynthaseandamylinreceptorlevelsbetween

7、dosagegroupsandthemodelgroupfP>O．05)．Therewerenosignificantdifferencesamonglow,mediumandhighdosagegroupsforalloftheaboveindexesf尸>0．05)．Conclusion：Inearlytype2DMcharacterizedbyelevatedlevelsofinsulinandamylininserum，LJPShashypoglycemiceffectsthatinvolverecoveryofisletcell