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《Nature Communications，2013--The effects of carbon dioxide and temperature on microRNA expression in Arabidopsis development.pdf》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、ARTICLEReceived13Dec2012
2、Accepted14Jun2013
3、Published31Jul2013DOI:10.1038/ncomms3145TheeffectsofcarbondioxideandtemperatureonmicroRNAexpressioninArabidopsisdevelopmentPatrickMay1,WillLiao2,3,YijinWu2,4,BinShuai5,W.RichardMcCombie6,MichaelQ.Zhang7,8&QiongA.Liu4ElevatedlevelsofCO2andtemperaturecanbot
4、haffectplantgrowthanddevelopment,butthesignallingpathwaysregulatingtheseprocessesarestillobscure.MicroRNAsfunctiontosilencegeneexpression,andenvironmentalstressescanaltertheirexpressions.Hereweidentify,usingthesmallRNA-sequencingmethod,microRNAsthatchangesignificantlyinexpressionbyeitherdoublingthe
5、atmosphericCO2concentrationorbyincreasingtem-perature3–6°C.Notably,nearlyallCO2-influencedmicroRNAsareaffectedinverselybyelevatedtemperature.UsingtheRNA-sequencingmethod,wedeterminestronglycorrelatedexpressionchangesbetweenmiR156/157andmiR172,andtheirtargettranscriptionfactorsunderelevatedCO2concen
6、tration.SimilarcorrelationsarealsofoundformicroRNAsactinginauxin-signalling,stressresponsesandpotentialcellwallcarbohydratesynthesis.OurresultsdemonstratethatbothCO2andtemperaturealtermicroRNAexpressiontoaffectArabidopsisgrowthanddevelopment,andmiR156/157-andmiR172-regulatedtranscriptionalnetworkm
7、ightunderlietheonsetofearlyfloweringinducedbyincreasingCO2.1BioinformaticsCore,LuxembourgCentreforSystemsBiomedicine,UniversityofLuxembourg,7avenuedesHauts-Fourneaux,Eschsur-AlzetteL-4362,Luxembourg.2TheDepartmentofAppliedMathematicsandStatistics,StonyBrookUniversity,StonyBrook,NewYork11794-3600,US
8、A.3ComputationalBiology,ColdSpringHarborLaboratory,1BungtownRoad,ColdSpringHarbor,NewYork11724,USA.4DepartmentofBiochemistryandCellBiology,StonyBrookUniversity,StonyBrook,NewYork11794,USA.5DepartmentofBiologicalSciences,WichitaStateUniversity,Wichita,Kansas67260,USA.6ColdSpringHarborLaboratory,1Bu
9、ngtownRoad,ColdSpringHarbor,NewYork11724,USA.7DepartmentofMolecularandCellularBiology,UniversityofTexasatDallas,Richardson,Texas75080,USA.8BioinformaticsDivision,CenterforSyntheticandSystemsBiology,TNLIST,Tsinghu
