The Molecular Basis of Temperature Compensation in the Arabidopsis Circadian Clock

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1、ThisarticleispublishedinThePlantCellOnline,ThePlantCellPreviewSection,whichpublishesmanuscriptsacceptedforpublicationaftertheyhavebeeneditedandtheauthorshavecorrectedproofs,butbeforethefinal,completeissueispublishedonline.Earlypostingofarticlesreducesnormaltimetopublicationbyseveralweeks.The

2、MolecularBasisofTemperatureCompensationintheArabidopsisCircadianClockWPeterD.Gould,aJamesC.W.Locke,b,c,dCamilleLarue,aMeganM.Southern,cSethJ.Davis,eShigeruHanano,eRichardMoyle,f,gRaechelMilich,fJoannaPutterill,fAndrewJ.Millar,d,h,1andAnthonyHalla,1aSchoolofBiologicalSciences,UniversityofLiv

3、erpool,LiverpoolL697ZB,UnitedKingdombDepartmentofPhysics,UniversityofWarwick,CoventryCV47AL,UnitedKingdomcDepartmentofBiologicalSciences,UniversityofWarwick,CoventryCV47AL,UnitedKingdomdInterdisciplinaryProgrammeforCellularRegulation,UniversityofWarwick,CoventryCV47AL,UnitedKingdomeMaxPlanc

4、kInstituteforPlantBreeding,CologneD-50829,GermanyfUniversityofAuckland,SchoolofBiologicalSciences,PrivateBag92019,Auckland,NewZealandgBotanyDepartment,SchoolofIntegrativeBiology,UniversityofQueensland,BrisbaneQld4072,AustraliahInstituteofMolecularPlantSciences,UniversityofEdinburgh,Edinburg

5、hEH93JH,UnitedKingdomCircadianclocksmaintainrobustandaccuratetimingoverabroadrangeofphysiologicaltemperatures,acharacteristictermedtemperaturecompensation.InArabidopsisthaliana,ambienttemperatureaffectstherhythmicaccumulationoftranscriptsencodingtheclockcomponentsTIMINGOFCABEXPRESSION1(TOC1

6、),GIGANTEA(GI),andthepartiallyredundantgenesCIRCADIANCLOCKASSOCIATED1(CCA1)andLATEELONGATEDHYPOCOTYL(LHY).TheamplitudeandpeaklevelsincreaseforTOC1andGIRNArhythmsasthetemperatureincreases(from17to278C),whereastheydecreaseforLHY.However,astemperaturesdecrease(from17to128C),CCA1andLHYRNArhythm

7、sincreaseinamplitudeandpeakexpressionlevel.At278C,adynamicbalancebetweenGIandLHYallowstemperaturecompensationinwild-typeplants,butcircadianfunctionisimpairedinlhyandgimutantplants.However,at128C,CCA1hasmoreeffectonthebufferingmechanismthanLHY,asthecca1an