吡格列酮对大鼠脑出血后aqp4表达的影响

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1、中文摘要目的观察吡格列酮对大鼠脑出血后AQP4表达的影响，探讨吡格列酮对大鼠脑出血后神经保护作用。方法将36只成年雄性Wistar大鼠随机分为假手术组、脑出血组、吡格列酮组，每组12只。建立大鼠白体血脑出血模型，模型成功后，吡格列酮组大鼠分别于术后即刻、术后24小时、术后48小时给予吡格列酮(2．5mg／kg)灌胃，假手术组及脑出血组则给予同量生理盐水灌胃。每组末次给药24小时后处死大鼠，半定量逆转录聚合酶链反应(reversetranscription—polyrmerasechainreaction，RT—PCR)检测

2、脑组织AQP4mRNA的表达，Western—blot检测脑组织AQP4蛋白的表达。结果RT—PCR显示，假手术组AQP4mRNA含量(0．3508±0．0413)最低，脑出血组AQP4mRNA含量(0．8536±0．0371)较假手术组明显增高(P=O．00，P

3、升高(P=O．00，P

4、racerebralhemorrhageinrats．ExploretheroleofpioglitazoneneuroprotectiveafterintracerebralhemorrhageinratS．Methods36ad．ultmaleWistarratswererandomlydividedintothesham—operatedgroup，intracerebralhemorrhagemodelgroupandpioglitazonegroup(N=12)．Intracerebralhemorrhagemo

5、delswereestablished．Aftermodelingthepioglitazonegroupratsweregivenpioglitazone(2．5mg／kg)every24hours(i．e．Immediately,24-htimepoint，48一htimepoint)．Otherratsweregiventhesameamountofsaline．24huorsafterthelastadministration，allofratsweresacrificed．Western-blotandRT-PC

6、RwereusedtodetecttheexpressionofAQP4proteinsandmRNAinthebraintissues，respectively．ResultsAmongallthegroups，theAQP4mRNAofthesham-operatedgroupshowedthelowestlevel(0．3508+0．0413)．theAQP4mRNAoftheintracerebralhemorrhagemodelgroup(0．85364-0．0371)werehighestthanthesham

7、-operatedgroup(P=O．00，P

8、significantlyintheintracerebralhemorrhagemodelgroup(O．98274-0．0947)(P=0．00，P<0．05)，buttheexpressionsofAQP4proteinsinthepioglitazonegroup(0．79724-0．0515)