人胎盘绒毛膜间充质干细胞在大鼠急性心肌梗死修复中的作用的实验分析

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1、中文摘要人胎盘绒毛膜间充质干细胞在大鼠急性心肌梗死修复中的作用的实验研究表明，CMSCs细胞在大鼠心梗区分化并表达CD31及Ⅷ因子；6.心超结果提示，各组+相比GFP的CMSCs细胞移植组心脏左室舒张末内径（LVEDd）、左室收缩末内径(LVEDs)明显减小，而心室射血分数(EF)、缩短分数（FS%)则明显升高，心功能明显改善，实验结果差异有统计学意义（P＜0.05）。结论：（1）CMSCs具有和BMSCs相似的分化潜能，同时具有良好的体外成血管能力，以及较强的体内毛细血管内皮细胞分化能力；（2）经开胸心脏梗死区

2、及边缘区行CMSCs直接移植对大鼠急性心梗死后心功能恢复效果显著；（3）CMSCs细胞可在大鼠心脏梗死区分化为内皮细胞，参与局部毛细血管新生，从而改善大鼠心功能，此为CMSCs细胞参与大鼠急性心梗修复的可能机制之一。关键词：人胎盘绒毛膜间充质干细胞心肌梗死细胞移植修复作者：刘磊指导老师：郑世营II人胎盘绒毛膜间充质干细胞在大鼠急性心肌梗死修复中的作用的实验研究英文摘要ExperimentalStudyonHumanPlacentalChorionicMesenchymalStemCellsRepairAcuteM

3、yocardialInfarctioninRatAbstractObjecive:Humanplacentachorionicmesenchymalstemcells(CMSCs)canbeeasilyexpandedinpropercultureconditionsinvitro，havingthepotentialtodifferentiateintovariouscelltypes,andeasytoformcapillarylikestructure.2weeksafterthemyocardialinf

4、arctionmodelofratswasestablished,humanCMSCswereinjectedintomyocardialinfarctionareaandedgeareaoftheleftventricularduringthoracotomy,toobserveitseffectonratsheartfunctionimprovement,andtofurtherstudyonthepossiblemechanism.Methods:MSCsisolatedfrommincedchoriont

5、issuesofmatureplacentawereculturedinvitro,afterbeenexpanded,thephenotypeofCMSCsweredetectedbytheFlowCytometryanalysis;underdifferentinductionconditions,CMSCswereinducedtodifferentmesodermallineagesandspecificdyewasadoptedtodeterminetheirdifferentiationpotenti

6、al.Capillary-LikeTubeFormationAssaywasadoptedtoshowtheabilityofCMSCsofformingthecapillary-likestructure.Themyocardialinfarctionmodelwasestablishedbyligaturingtheleftanteriordescendingcoronaryarteryinrats.2weekslater,theGFPpositiveCMSCsandBMSCscellsweretranspl

7、antedintomyocardialinfarctionareaandedgeareaoftheleftventricularduringthoracotomy.Ratsweredividedintofourgroups:A(controlgroup),thoracotomywascarriedoutbutnotligation;GroupB(modelgroup),surgicalligaturingleftanteriordescendingcoronaryartery.GroupC(BMSCsinject

8、iongroup),BMSCscellswereinjectedintotheedgeandthecardiacinfarctionarea.GroupD(CMSCsinjectiongroup),CMSCscellswereinjectedintotheedgeandthecardiacinfarctionarea.4weeksaftertransplantation,