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1、Downloadedfromrspb.royalsocietypublishing.orgonOctober10,2010AncientwolflineagesinIndiaDineshK.Sharma,JesusE.Maldonado,YadrendradevV.JhalaandRobertC.FleischerProc.R.Soc.Lond.B2004271,S1-S4doi:10.1098/rsbl.2003.0071Supplementarydata"DataSupplement"http://rspb.royalsocietypublishing.or
2、g/content/suppl/2009/01/23/271.Suppl_3.S1.DC1.htmlEmailalertingserviceReceivefreeemailalertswhennewarticlescitethisarticle-signupintheboxatthetopright-handcornerofthearticleorclickhereTosubscribetoProc.R.Soc.Lond.Bgoto:http://rspb.royalsocietypublishing.org/subscriptionsThisjournalis
3、©2004TheRoyalSocietyDownloadedfromrspb.royalsocietypublishing.orgonOctober10,2010pallipeswolvesfromtheMiddleEast(n=40)fallwithinthewolf–dogclade(Tsudaetal.1997;Vilaetal.1997,1999;Savolainenetal.2002).WeusemtDNAsequencestoassessthephylogeographyofwolvesfromtheIndiansubcontinentandthei
4、rrelationshipstootherwolfsub-Ancientwolflineagesspeciesandcanids.inIndia2.MATERIALANDMETHODS(a)SamplesandsequencesDineshK.Sharma1,JesusE.Maldonado2,WeobtainedmtDNAcontrolregion(CR)sequencesfor45C.l.YadrendradevV.Jhala1andRobertC.Fleischer2,3*pallipesfromthroughoutmostoftheircurrentra
5、ngeinthesubconti-nentand23C.l.chancofromthewesternandcentralHimalayas1WildlifeInstituteofIndia,Postbox18,Dehradun248001,India(figure1b;electronicAppendixB,availableonTheRoyalSociety’s2GeneticsProgram,SystematicBiologyDepartment,NationalMuseumPublicationsWebsite).Mostsampleswereobtain
6、edfromanongoingofNaturalHistory,SmithsonianInstitution,3001ConnecticutAvenue,studyattheWildlifeInstituteofIndia,but20sequenceswereNW,Washington,WA20008-0551,USAobtainedfrommuseumspecimens.Wecomparedthesesequences3DepartmentofBiologicalSciences,UniversityofDurham,SouthRoad,withallofth
7、ewolfanddogsequencescurrentlyavailableinGenBankDurhamDH13LE,UK(254haplotypesfrom453differentsequencesfrommorethan700*Authorforcorrespondence(fleischer.robert@nmnh.si.edu).individuals),whichinclude40individualC.l.pallipesfromtheMiddleEast(AfghanistantoTurkey)and22C.l.chancofromMong-Re
8、cd12.05.03;A