肝素对脓毒症大鼠肠上皮细胞通透性与occludin蛋白表达的分析

《肝素对脓毒症大鼠肠上皮细胞通透性与occludin蛋白表达的分析》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、英文论著摘要TheeffectofHeparinonratsintestinalepithelialcellpermeabilityandexpressionofOccludinexresslonccluelinobjectiveObservesepsisratintestinalepithelialtightjunctionstypicalperformance，Occludinproteinexpressioninratintestinalepithelialcellsunderelectronmicroscopetoverifywhetherintestinalepitheli

2、alpermeabilitychangesandwhetherheparinhasprotectiveeffectonacuteintestinalepithelialcellspermeabilityMethodsChoose60healthSPFSDrats，nomattermaleorfemale，weightfrom1809to2209wererandomlygroupedintonormalsalinegroup，sepsis，sepsisheparingroup．Salinegroup：(n220)：intraperitonealinjectionofanequalvol

3、ume(1m1)saline，sepsisgroup：(n220)：asingleintraperitonealinfusionoflethalLPS，10mg／kg；sepsisheparingroup：(n220)：HeparinIntervention(1OOU／kg)aftertwohoursLPS(1Omg／kg)modelbuilt．Theratsweresacrificedbycervicaldislocationaftersixhours，routineiodophordisinfectionfortheabdominalskin，steriledopentheabd

4、ominal，observetheinflammatoryconditionchangesinintra-abdominalbynakedeye，pancreaschange，boweldilatation，congestion，edema；observethedegreeofintestinalmucosalinjuryofilealbiopsyspecimensunderlOOxlightmicroscope；useelectronmicroscopytoobservethestructureofintestinalepithelialcelltightjunctionsandt

5、hechanges；useimmunehistochemicalassaytoseetheratintestinalepithelialtightjunctionproteinoccludindistributionandexpressionchanges．MethodsIntraperitonealchanges：Thesalinegrouphadnosignificantsignsofinflammation，bowelhadnoedemaorcongestion；sepsisandseptic+heparingroupwereobservedhadvaryingdegreeso

6、fascites，boweledema，hyperemia．Incontrasttheboweledemaofsepsisgroupismoresignificant．Intestinalmucosalpathology：thesalinegroupnoobviousintestinaldamage，howevervarydegreesileumdamageoftheintestinalmucosainsepsisandseptic+heparingroupwereobserved．thesalinegroup，thesepsisgroupshowedintestinalmucosa

7、inflammatorycellinfiltration，interstitialedema，irregularvilli，mucosalerosionnecrosis，P<0．05；thesepsis+heparingroupshowedintestinalinflammatorycellinfiltration，intestinaledema，villussubepithelialspaceexpanded，butwasnotassevereasthe