内毒素对nrk52e细胞生长及细胞间缝隙连接功能的影响

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1、第31卷第2期中山大学学报(医学科学版)V01．3lNo．22010年3月JOURNALOFSUNYAT—SENUNIVERSITY(MEDICALSCIENCES)Mar．2010内毒素对NRK52E细胞生长及细胞问缝隙连接功能的影响位静，甘小亮，罗晨芳，李晓芸，陈景辉，黑子清(中山大学附属第三医院麻醉科，广东广州510630)摘要：【目的】研究不同浓度内毒素(LPS)对NRK52E细胞生长和细胞问缝隙连接功能的影响。【方法】体外培养NRK52E细胞，随机分为对照组及LPS处理组，LPS处理组分别用5个不同浓度的L

2、PS(10ng／mL、50ng／mL、100ng／mL、500ng／mL、1000ng／mL)作用24h。采用四甲基偶氮唑盐法检测NRK52E细胞的生长；细胞荧光免疫示踪法测定NRK52E细胞间缝隙连接传递的功能；WesternBlotting测定对照组、LPS10ng／mL组和LPS100ng／mL组Cx43蛋白的表达。【结果】①与对照组和LPS10ng／mL组相比．LPS50ng／mL、100ng／mL、500ng／mL及l000ng／mL组细胞生长明显降低(P<0．01)。②与对照组相比，LPS100ng／mL

3、、500ng／mL及1000ng／mL组细胞间缝隙连接功能明显降低(P<0．01)。③内毒素浓度与NRK52E细胞生长和细胞间缝隙连接传递数目呈负相关r=一0．941。一0．872，P<0．01)。④与对照组比较，LPS10ng／mL组和LPS100ng／mL组的Cx43蛋白表达明显降低(P

4、)02．0249—04EfectsofEndotoxinonGrowthActivityandGapJunctionofNRK52ECellsWEIJing，GANXiao—liang，LUOChen—fang，LIXiao·yun，CHENJing—hui，HEIZi—qing(DepartmentofAnesthesiology，TheThirdAffiliatedHospital，SunYat—senUniversity，Guangzhou510630，China)Abstract：【Objective】Thi

5、sstudywasdesignedtoobservetheeffectsofendotoxin(LPS)onthegrowthactivityandgapjunction(GJ)ofNRK52Ecells．【Methods】TheNRK52EcellsweredividedintocontrolgroupandLPSgroups，andtheNRK52EcellsinLPSgroupsweretreatedwithLPS10ng／mL，50ng／mL，100ng／mL，500ng／mL，andl000ng／mLfor

6、24hrespectively．TheNRK52EcellsgrowthactivitywasmeasuredthroughM1Trmethod，andthefunctionofgapjunctionofNRK52Ecellswasmeasuredthroughthemethodoffluoroimmunoassay．Theproteinexpressionofconnexin43(Cx43)incontrolgroup，LPS10ng／mLgroup，andLPS100ng／mLgroupwerealsodeter

7、minedbyWesternblotting．【Results】①ComparedwithcontrolgroupandLPS10ng／mLgroup，TheNRK52EcellsgrowthactivitydecreasedsignificantlyinLPS50ng／mL，100ng／mL，500ng／mL，and1000ng／mLgroups(P<0．01)．②Comparedwithcontrolgroup，thefunctionofGJdecreasedsignificantlyinLPS100ng／mL，

8、500ng／mL，and1000ng／mLgroups(P<0．O1)．⑧TherewerenegativecorrelationsamongtheconcentrationofLPSandNRK52EcellsgrowthactivityandGJfunctionrespectively(r=-0．941，一0．872，P<0．01)．④Co