资源描述：
《sirna沉默traf6基因对食管癌ec109细胞增殖及凋亡的影响》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、汕头大学医学院硕士学位论文TRAF6siRNAandNegativeControlsiRNA;2.24haftertransfection,cellsweredigestedbyRNAisoReagenttoextracttotalRNA;RT-PCRwascarriedouttoconfirmthetransfectioneffect;3.Aftertransfectionfor24h,adherentcellswerecollected,washedwithPBS,centrifugedandthensolublesubcellularpro
2、teinswereextractedbyproteinextractionkit.WestenBlotwasusedtoexploitTRAF6andP65proteinsexpressionineachgroups.4.TransfectedbysiRNAfor24h,adherentcellswerecollected,washedwithPBS,centrifuged,fixedwith4%polylysine,andthendealwith0.5%tritonX100.Afterantibodyincubationfluorescence
3、intensityofTRAF6wasdetectedbyflowcytometry;5.TransfectedbysiRNAfor24h,thecellproliferationwasmeasuredbyMTTassay,andapoptosiswastestedbyflowcytometry.6.ExperimentaldatadealedwithSPSS13.0statisticalanalysissoftware.Results:1.AsiRNAwhichcouldeffectivelyblockTRAF6geneexpressionwa
4、sidentifyed,andtheoptimumconcentrationwasfound.2.ItshowseffectivelyinterferenceofTRAF6genebyRT-PCR,WesternBlotandflowcytometry.3.AfterTRAF6genewasblocked,itshowsthatP65proteinexpressedlowerinnuclearextractbywesternblot;4.ThecellularmorphologyofEC109cellshasnosignificantchange
5、betweencellstreatedwithTRAF6siRNAandcellswithNegativeControlsiRNA.TRAF6genedoesn’taffecttheproliferationofEC109cells,butcanaffectapoptosisofEC109cells.Conclusions:TRAF6genemayplayanimportantroleinindevelopmentandprogressionofhumanesophagealcancer,andthemechanismmayinduceexces
6、siveactivationofNF-κBinEC109cellstocarryouttheinhibitingapoptosisfunctions.Keywords:Apoptosis,RNAi,SignalTransduction,TRAF6,NF-κBIV汕头大学医学院硕士学位论文缩略词表缩略词英文中文AcrAcrylamide丙烯酰胺APAmmoniumpersulfate过硫酸铵BSAbovineserumalbumin牛血清白蛋白bpBasepair碱基对cDNAComplementarydeoxyribonucleicacid互补脱
7、氧核糖核酸DEPCdiethypyrocarbonate焦碳酸二乙酯DMSODimethylSulfoxide二甲基亚砜DMEMDulbecco’smodifiedeagle’smedium达尔伯克改良伊格尔培养基dNTPDeoxynucleotidetriphosphate脱氧核苷三磷酸FBSfatalbovineserun胎牛血清TRAFstumornecrosisfactorreceptorassociatedfactor肿瘤坏死因子受体相关因子TNFRTumornecrosisfactorreceptor肿瘤坏死因子受体TRANCETNF
8、-relatedActivation-InducedCytokine肿瘤坏死因子相关激活诱导因子NF-κBnucleartranscri
