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1、第59卷第2期化工学报Vol159No122008年2月JournalofChemicalIndustryandEngineering(China)February2008研究论文絮凝酿酒酵母的构建及其发酵特性1122211王付转,沈微,饶志明,诸葛斌,詹晓北,方慧英,诸葛健(1江南大学工业生物技术教育部重点实验室;2江南大学生物工程学院工业微生物研究中心,江苏无锡214122)摘要:把S1cerevisiaeW30321A的絮凝基因FLO1和G418抗性基因kanMX接入质粒pYX212,构建重组质粒pYX2FLO2kan并转化S1cerevisi
2、aeZWA46,得到了较强絮凝能力且传代稳定的重组菌ZWA462F2。考察其絮凝能力及发酵特性,实验结果表明:该重组菌在生长稳定期前期启动絮凝,与葡萄糖的耗竭非耦联;在初始pH215～610范围内絮凝值无明显改变;乙醇产量最高达到816%(体积),乙醇对葡萄糖的转化率达到其理论转化率的8918%。重组菌ZWA462F2良好的絮凝性能有利于从发酵液中分离细胞和细胞回用,在燃料乙醇工业生产中有一定的应用价值。关键词:絮凝;转化;发酵;燃料乙醇中图分类号:TQ92文献标识码:A文章编号:0438-1157(2008)02-0437-06Constructi
3、onofaflocculatingyeastforfuelethanolproduction1122WANGFuzhuan,SHENWei,RAOZhiming,ZHUGEBin,211ZHANXiaobei,FANGHuiying,ZHUGEJian(1KeyLaboratoryofIndustrialBiotechnologyofMinistryofEducation2ResearchCenterofIndustrialMicroorganisms,SchoolofBiotechnology,JiangnanUniversity,Wuxi2141
4、22,Jiangsu,China)Abstract:TheexpressionvectorpYX212harboringtheflocculationgeneFLO1fromS1cerevisiaeW30321AandtheG418resistancegenekanMXwastransformedintoS1cerevisiaeZWA461ThetransformantZWA462F2wasobtainedandshowedstrongandstableflocculationability1Otherpropertiesoftherecombina
5、ntstrainwerealsostudied1Theresultsdemonstratedthattheonsetflocculationwasintheearlystationaryphase,notcoincidentwithglucosedepletionintheculturemedium1Moreover,theflocculationabilityofthetransformantshowednodifferenceatinitialpHrangingfrom215to610,andcouldachievethemaximumethan
6、olconcentrationof816%(vol.)withanethanolyieldonglucoseof8918%ofthetheoreticalvalue1TheflocculationpropertyofthetransformantZWA462F2seemedtohavepotentialapplicationinthefuelethanolindustryduetotheeasinesstoseparateandreuseyeastcells.Keywords:flocculation;transformant;fermentatio
7、n;fuelethanol2007-06-06收到初稿,2007-08-16收到修改稿。Receiveddate:2007-06-06.联系人:诸葛健。第一作者:王付转(1973—),女,博士研Correspondingauthor:Prof.ZHUGEJian.E-mail:jzhuge@究生,讲师。sohu1com基金项目:国家自然科学基金项目(20676053);国家高技术Foundationitem:supportedbytheNationalNaturalScience研究发展计划项目(2006AA020103);江苏省青年科技创新人才Fo
8、undationofChina(20676053);theHigh2techResearchand(学术带头
