塞来昔布对胰腺癌细胞株sw1990增殖有干性标记物cd133表达的影响

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1、中文摘要塞来昔布对胰腺癌细胞株SWl990增殖及干性标记物CD瑚表达的影响目的观察环氧合酶-2(COX一2)选择性抑制剂塞来昔布对胰腺癌细胞株SWl990增殖及肿瘤干细胞标记物CD。。。表达的影响。方法取对数生长期的SWl990细胞，分组后分别加入不同浓度的单药吉西他滨、单药塞来昔布、塞来昔布联合吉西他滨进行培养，观察SWl990增殖抑制情况、CD。。。mRNA及表面分子CD。∞的表达变化。结果塞来昔布与吉西他滨(0．5P-mol／L)联合应用，对SWl990细胞的增殖抑制作用更为明显，与0．5lZmol／L吉西他滨组比较，差异均有显著性(P<0．05

2、)，与对应剂量的塞来昔布组比较，差异亦均有显著性(P<0．05)。塞来昔布组胰腺癌细胞株SWl990CD。ssmRNA的表达较对照组明显下调(P<0．05)，联合应用塞来昔布与吉西他滨(0．5umol／L)时，其下调作用较各浓度单药塞来昔布减弱，差异有显著性(P

3、；塞来昔布；吉西他滨；CD·∞；英文摘要TheinfluenceofCELECOXIBontheproliferationofpancreaticcancereelllineSWl990andtheexpressionofstemcellmarkerCDl33AbstractObjectiveObservationtheimpactofcyclooxygenase一2(cox-2)selectiveinhibitorcelecoxibonpancreaticcancercelllineSWl990proliferationandstemcellmark

4、erCDl33expression．MethodAfergrouped，theSWl990cellsinlogarithmicphasecultured、Vi也differentconcentrationsofgemcitabine，celecoxibandcelecoxibcombinedwithgemcitabine，respectively．ThenthesuppressionofSWl990proliferation，mRNAofCD,33andthechangesofsurfacemoleculeCDl33expressionwereobse

5、rved．ResuRCombinedcelecoxib谢mgemcitabine，theproliferationofSWl990cellsWasmoreobvious，comparedwithgemcitabinegroup(0．5umol／L)，thedifferencesweresignificant(P<0．05)，compared谢mthecorrespondingdosesofcelecoxibgroup，thedifferenceswerestatisticallysignificant(P<0．05)。Celecoxibgrouppan

6、creaticcancercelllineSWl990CDl33mRNAexpressioncompared晰t11thecontrolgroupWassignificantlylower(P

7、looxygenase一2(cox-2)selectiveinhibitorcelecoxibenhancedthegrowthinhibitionofpancreaticcancerinducedbygemcitabine，whichcanlowerstemcellsurfacemarkersCDl33mRNAexpressionsignificantly．Postgraduatestudent：LiuLi(Ontology)DirectedbyProf：Fang-ZhenShen[Keywords]pancreaticcancer；cancerst

8、emcells；celecoxib；gemcitabine；CDl33；目录引言⋯⋯⋯⋯⋯⋯⋯