光动力疗法对喉癌hep-2细胞迁移和侵袭的影响

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1、万方数据复旦大学硕士学位论文光动力疗法对喉癌HEp．2细胞迁移和侵袭的影响TheeffectsofphotodynamictherapyonmigrationandinvasionofHEp一2humanlaryngealcancercellline硕士类型：临床型医学硕士院系：复旦大学附属眼耳鼻喉科医院专业：耳鼻咽喉科学姓名：张焕康指导教师：肖宽林教授小组成员：何培杰李雯万方数据目录IIIIIIIMIlUllIIIIIIIIIlllllllUllIIIIIIIY2700979缩略语简表····························-····

2、························1中文摘要···························································2英文摘要···························································3前言·································-······-······················4日U舌················。················-····。。’······。·········

3、······4资料与方法·························································8结果······························································20讨t《≥······························································25结．}仑······························································27参考文献···

4、··············-········································28综述··-···························································33致谢······························································40万方数据复旦大学硕士毕业论文缩略语简表英文缩写英文全称PDTPhotodynamictherapyROSreactiveoxygenspecies9-HPbD9-hydroxyph

5、eophorbide0【GSHMMP．2MMP．9ECMERKMEKLCHEp-2Glutathionematrixmetalloproteinases-2matrixmetalloproteinases-9extracellularmatrixextracellularregulatedproteinkinasesmitogen-activatedERK-regulatingkinaselaryngealcancerHEp一2humanlaryngealcancercelllineH2DCFDA2’，7'-dichlorodihydrofluores

6、ceindiacetateGAPDHMTTPBSSPSSglyceraldehyde··3··phosphatedehydrogenase3-(4．5一Dimethylthiazol一2一y1)·-2,5··diphenyltetrazoliumbromidePhosphateBufferedSalineStatisticalProductandServiceSolutionsDMSODimethylsulfoxide1中文全称光动力疗法活性氧9．羟基脱镁叶绿酸甲酯0t谷胱甘肽基质金属蛋白酶．2基质金属蛋白酶．9细胞外基质细胞外调节蛋白激酶丝裂原活化的

7、细胞外信号调节激酶之调节激酶喉癌HEp．2人喉鳞癌细胞株2’，7’．二氯二氢荧光素二乙酯甘油醛．3．磷酸脱氢酶3．(4，5．二甲基噻唑．2)-2，5．二苯基四氮唑溴盐磷酸盐缓冲液统计产品与服务解决方案软件二甲基亚砜万方数据复旦大学硕士毕业论文光动力疗法通过活性氧抑制喉癌HEp-2细胞的迁移和侵袭中文摘要目的：光动力疗法(PhotodynamicTherapy,PDT)是借助某种特定的药物(称为光敏剂)进入患者体内后，富集于生长异常的组织(如肿瘤)，光敏剂经一定波长光的辐照后发生光动力敏化反应而产生活性氧(reactiveoxygenspecies，RO

8、S)，导致生物大分子光氧化失活，并由此造成细胞损伤以达到破坏目标病变组织的目的。我们通过本实验