大菱鲆早期性别分化与性别相关snp位点筛选分析

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1、上海海洋大学硕士毕业论文StudiesOnEarlySexDifferentiationAndSex-associatedSNPsScreeningOfTurbots（ScophthalmusMaximusL.）ABSTRACTTheturbot(ScophthalmusmaximusL.)isaflatfishofgreatcommercialvalue,whereastrongsexualdimorphismexistsforgrowthrate.Inthisstudy,byusinghaematoxylin-eosi

2、nstainingofhistologysectionofthegonadsandmacroscopicexaminationfistly,phenotypicsexofjuvenileindividualsfrom3to5monthsagewereidentified.Thensexratioof4022individualswithin37familiesat4monthswereanalyzedbothatfamilyandpopulationlevel.Meanwhile,2254individualsfrom20

3、familiesamong37familieswererandomlyselectedforanalysisofsexualdimorphisminbodyweight.CandidateSNPlocicomefromlarge-scalehigh-throughputsexspecialtranscriptome454sequencingsystemofturbot(ScophthalmusmaximusL.).AccordingtothecandidateSNPloci,primersweredesignedtotes

4、tandverify.AndbyuseofMassArray(MALDI-TOFMSArray),ahigh-throughputgenotypingtechnologyforSNPs,398sexassociatedcandidateSNPswerescreenedandverifiedwith20malesandfemalesat8monthsage.Thentheother35sex-associatedcandidateSNPsof6contigswereverifiedandconductedhaplotypes

5、analysis.Themainresultsareasfollows:1.Bydissectingindividualsat4monthsagedirectly,significantshapedifferenceofgonadsbetweenmaleandfemaleswereobserved.Females’wereshortrod-likewhilemalesaslenderline-like.Then,sexofmost4monthsageindividualscouldbeidentifiedbymacrosc

6、opicexamination.Fewindividualsthatshapeofgonadsshowednosignificantdifferencebetweensexes,stillshouldbeidentifiedbyuseofhaematoxylin-eosinstainingofhistologysectionoftheirgonads.Andashapedifferenceofgonadswasobservedbetween4and6monthsageindividuals,whichsuggestedth

7、atshapeofturbotgonadswascontinuouslychangingduringearlystage.2.Phenotypicsexof4022individualscomposedof37familiesat4monthsagewereidentifiedandcounted.Thestatisticalanalysisshowedthatthenumberoffemaleandmalewere2426and1596,respectively,whichreflectedasignificantdif

8、ference(P<0.01)III万方数据上海海洋大学硕士毕业论文insexratiotothehypothesisofabalancedsexratio(50%males/50%females).Andatfamilylevel,therewere19familiesamong37familiese