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ID:34154550
大小:4.62 MB
页数:61页
时间:2019-03-02
《cd4+cd25+调节性t细胞在狼疮性肾炎中的作用及霉酚酸酯对其的影响》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、分类号——学校代码——密级学号CD4+CD25+调节性T细胞在狼疮性肾炎中的作用及霉酚酸酯对其的影响CD4+CD25+TregcellsinLNandthee舵ctofMMFonthe仔equencyofTregs.研究生姓名指导教师姓名、职称学科专业研究方向湖南师范大学学位评定委员会办公室二零一三年五月摘要目的:观察CD4+CD25+调节性T细胞及细胞因子在狼疮肾炎(LN)患者中表达的变化及予霉酚酸酯对其的影响。为霉酚酸酯用于狼疮性肾炎及其他自身免疫性疾病的治疗提供新的理论依据。方法:用流式细胞仪检测LN患者外用血CD4+CD25+T细胞的百分率,酶联免疫吸附法测定细胞因
2、子IL.2,IL.10的表达。予激素加霉酚酸酯诱导治疗6个月后再次用流式细胞仪检测LN患者外用血CD4+CD25+T细胞的百分率,酶联免疫吸附法测定细胞因子IL.2,IL.10的表达。并与激素加环磷酰胺组治疗进行对比,以观察霉酚酸酯对CD4+CD25+调节性T细胞的影响。结果:①病例组CD4+CD25+调节性T细胞数量显著低于正常对照组,且伴有IL.2含量增高及IL.10含量降低,差异有统计学意义(P<0.05)。②病例组予霉酚酸酯(MI、伍)治疗后CD4+CD25+调节性T细胞数量较前明显升高,几.2含量下降,IL.10表达升高,差异均有统计学意义(P3、例组予环磷酰胺(CTX)治疗后CD4+CD25+调节性T细胞数量、IL.10表达较前无明显变化,差异无统计学意义(P>O.05),但IL一2含量下降,差异仍均有统计学意义(P4、estigateme舶quenCyofCD4+CD25+regulato巧TcellsmLNpatiemsandthee恐Ctof啪onme舶quencyofTregs.AndproVidememeo巧foundationsaboutMM田forLupusnephritis觚dotherautoiInmunedisease.Methods:111e舶quencyofCD4+CD25+TregcellsiIltheperipheralbloodwasdetectedbynowcytomet巧a11dtheexpressionofcytokille几一2a11d几一10wasd5、etectedbyELISAbomatbaSelineanda舭r6monthsmer印yofM№ThetherapyofCTXalsotake6months嬲acontrolgrolJpwith加洒Results:①ThepercentageofCD4+CD25+regulato巧TcellswassignificantlydecreaSedi11patientsthanillcontrols(p6、ssignific趾tlydecreasediIlpatientsWhilet11eexpressionofIL一2wassigm丘c觚tlyincreaSedi11patientsthanincontrols(p7、edidnotsi印ific枷lyalterthe锄。嘶ofTregsandm-lO(P>O.05).IIIConclusion:①TheexpressionofCD4+CD25+Tregcellinmepe却heralbloodw嬲lowintheLupusn印11ritis.②MⅣⅢcouldselectivelyiI“bitmeproliferationofTandBlymphocytes,i11creasethemJrIlberofCD4+CD25+Tregs③MⅣⅢcoulddecreasetllen啪b
3、例组予环磷酰胺(CTX)治疗后CD4+CD25+调节性T细胞数量、IL.10表达较前无明显变化,差异无统计学意义(P>O.05),但IL一2含量下降,差异仍均有统计学意义(P4、estigateme舶quenCyofCD4+CD25+regulato巧TcellsmLNpatiemsandthee恐Ctof啪onme舶quencyofTregs.AndproVidememeo巧foundationsaboutMM田forLupusnephritis觚dotherautoiInmunedisease.Methods:111e舶quencyofCD4+CD25+TregcellsiIltheperipheralbloodwasdetectedbynowcytomet巧a11dtheexpressionofcytokille几一2a11d几一10wasd5、etectedbyELISAbomatbaSelineanda舭r6monthsmer印yofM№ThetherapyofCTXalsotake6months嬲acontrolgrolJpwith加洒Results:①ThepercentageofCD4+CD25+regulato巧TcellswassignificantlydecreaSedi11patientsthanillcontrols(p6、ssignific趾tlydecreasediIlpatientsWhilet11eexpressionofIL一2wassigm丘c觚tlyincreaSedi11patientsthanincontrols(p7、edidnotsi印ific枷lyalterthe锄。嘶ofTregsandm-lO(P>O.05).IIIConclusion:①TheexpressionofCD4+CD25+Tregcellinmepe却heralbloodw嬲lowintheLupusn印11ritis.②MⅣⅢcouldselectivelyiI“bitmeproliferationofTandBlymphocytes,i11creasethemJrIlberofCD4+CD25+Tregs③MⅣⅢcoulddecreasetllen啪b
4、estigateme舶quenCyofCD4+CD25+regulato巧TcellsmLNpatiemsandthee恐Ctof啪onme舶quencyofTregs.AndproVidememeo巧foundationsaboutMM田forLupusnephritis觚dotherautoiInmunedisease.Methods:111e舶quencyofCD4+CD25+TregcellsiIltheperipheralbloodwasdetectedbynowcytomet巧a11dtheexpressionofcytokille几一2a11d几一10wasd
5、etectedbyELISAbomatbaSelineanda舭r6monthsmer印yofM№ThetherapyofCTXalsotake6months嬲acontrolgrolJpwith加洒Results:①ThepercentageofCD4+CD25+regulato巧TcellswassignificantlydecreaSedi11patientsthanillcontrols(p6、ssignific趾tlydecreasediIlpatientsWhilet11eexpressionofIL一2wassigm丘c觚tlyincreaSedi11patientsthanincontrols(p7、edidnotsi印ific枷lyalterthe锄。嘶ofTregsandm-lO(P>O.05).IIIConclusion:①TheexpressionofCD4+CD25+Tregcellinmepe却heralbloodw嬲lowintheLupusn印11ritis.②MⅣⅢcouldselectivelyiI“bitmeproliferationofTandBlymphocytes,i11creasethemJrIlberofCD4+CD25+Tregs③MⅣⅢcoulddecreasetllen啪b
6、ssignific趾tlydecreasediIlpatientsWhilet11eexpressionofIL一2wassigm丘c觚tlyincreaSedi11patientsthanincontrols(p7、edidnotsi印ific枷lyalterthe锄。嘶ofTregsandm-lO(P>O.05).IIIConclusion:①TheexpressionofCD4+CD25+Tregcellinmepe却heralbloodw嬲lowintheLupusn印11ritis.②MⅣⅢcouldselectivelyiI“bitmeproliferationofTandBlymphocytes,i11creasethemJrIlberofCD4+CD25+Tregs③MⅣⅢcoulddecreasetllen啪b
7、edidnotsi印ific枷lyalterthe锄。嘶ofTregsandm-lO(P>O.05).IIIConclusion:①TheexpressionofCD4+CD25+Tregcellinmepe却heralbloodw嬲lowintheLupusn印11ritis.②MⅣⅢcouldselectivelyiI“bitmeproliferationofTandBlymphocytes,i11creasethemJrIlberofCD4+CD25+Tregs③MⅣⅢcoulddecreasetllen啪b
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