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ID:34150762
大小:2.58 MB
页数:45页
时间:2019-03-04
《前列地尔后处理对大鼠缺血再灌注心肌地影响》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、前列地尔后处理对大鼠缺血再灌注心肌的影响硕士研究生学位论文前列地尔后处理对大鼠缺血再灌注心肌的影响摘要目的:研究前列地尔后处理对大鼠心肌缺血再灌注模型的血流动力学、血清cTnI水平、心肌Bcl.2、Bax蛋白表达和心肌超微结构的影响,初步探讨其对缺血再灌注心肌损伤的保护作用及可能机制,为心肌损伤的治疗提供一定的实验依据。方法:将健康SpragueDawley雄性大鼠32只,随机分为4组:假手术组(A组)、模型组(B组.)、低剂量组(C组)、高剂量组(D组),每组8只。A组只穿线不结扎冠状动脉左前降支(LAD),B、C、D组结扎LAD,制作在体缺血
2、再灌注心肌模型,缺血30min,再灌注60min。采用体表心电图出现心肌缺血改变作为判断实验模型制备成功标准。B组在复灌前5min,静脉注射0.9%生理盐水0.25ml/kg;C组在复灌前5min,静脉注射前列地尔0.5gg/kg;D组在复灌前5min,静脉注射前列地尔lgg/kg。记录基础值(T1)、缺/血30min(T2)、再灌注30min(T3)和再灌注60min(T4)各时点大鼠心率(HR)、平均动脉压(MAP)和心率收缩压乘积(RPP);在T】、T2和T4时点取血标本用酶联免疫吸附法检测血清心肌肌钙蛋白I(cTnI);用免疫组化法检测大
3、鼠心肌Bcl.2、Bax蛋白表达;通过光镜和电镜观察心肌组织病理及超微结构的变化。结果:(1)与T1比较,C、D组在T4时点MAP和RPP值明显下降,差异有统计学意义(P0.05)。在T4时点,与B组比较,C、D组cTnI水平降低(P4、)与A组比较,B、C、D组Bcl.2、Bax蛋白表达增加,差异有统计学意义(P5、在;(2)前列地尔后处理可降低大鼠血清cTnl水平,上调Bcl.2蛋白表达、下调Bax蛋白表达,心肌细胞凋亡减少,提示前列地尔对缺血再灌注心肌有保护作用,且高剂量组效果更佳。关键词:心肌缺血再灌注;前列地尔;后处理;心肌肌钙蛋白I;Bcl.2:BaxEl砸'ECTOFALPROS呦ⅡJPOSTCONDmoNINGONMYoCARDIALISCHEM口已▲.REPERFUSIONIN.n瓜Y矾TATSAbstractObjective:ToinvestigatetheeffectofAlprostadilpostconditioningonmyoc6、ardialischemia-referfusioninjuryinrats.Methods:Theratmodelsofmyocardialischemia—reperfusionwasestablishedwithocclusionoftheleftanteriordescendingcoronaryartery(LAD)for30minandreperfusionfor60min.32ratsweredividedinto4groups,groupAofthemwastakenasshamoperationgroup,groupBcontr7、olgroup(ischemia30min,reperfusion60min),groupC(Alprostadil0.5}tg/kg,iv,5minpriortoreperfusion),andgroupD(Alprostadil1I_tg/kg,iv,5minpriortoreperfusion).ThechangesofhemodynamicsparameterandserumcardiactropininI(cTnI)weredetected.Thepathologicalchangesofmyocardiumwereobservedwi8、thmicroscopeandtransmissionelectronmicroscope.Theheartswereremovedfo
4、)与A组比较,B、C、D组Bcl.2、Bax蛋白表达增加,差异有统计学意义(P5、在;(2)前列地尔后处理可降低大鼠血清cTnl水平,上调Bcl.2蛋白表达、下调Bax蛋白表达,心肌细胞凋亡减少,提示前列地尔对缺血再灌注心肌有保护作用,且高剂量组效果更佳。关键词:心肌缺血再灌注;前列地尔;后处理;心肌肌钙蛋白I;Bcl.2:BaxEl砸'ECTOFALPROS呦ⅡJPOSTCONDmoNINGONMYoCARDIALISCHEM口已▲.REPERFUSIONIN.n瓜Y矾TATSAbstractObjective:ToinvestigatetheeffectofAlprostadilpostconditioningonmyoc6、ardialischemia-referfusioninjuryinrats.Methods:Theratmodelsofmyocardialischemia—reperfusionwasestablishedwithocclusionoftheleftanteriordescendingcoronaryartery(LAD)for30minandreperfusionfor60min.32ratsweredividedinto4groups,groupAofthemwastakenasshamoperationgroup,groupBcontr7、olgroup(ischemia30min,reperfusion60min),groupC(Alprostadil0.5}tg/kg,iv,5minpriortoreperfusion),andgroupD(Alprostadil1I_tg/kg,iv,5minpriortoreperfusion).ThechangesofhemodynamicsparameterandserumcardiactropininI(cTnI)weredetected.Thepathologicalchangesofmyocardiumwereobservedwi8、thmicroscopeandtransmissionelectronmicroscope.Theheartswereremovedfo
5、在;(2)前列地尔后处理可降低大鼠血清cTnl水平,上调Bcl.2蛋白表达、下调Bax蛋白表达,心肌细胞凋亡减少,提示前列地尔对缺血再灌注心肌有保护作用,且高剂量组效果更佳。关键词:心肌缺血再灌注;前列地尔;后处理;心肌肌钙蛋白I;Bcl.2:BaxEl砸'ECTOFALPROS呦ⅡJPOSTCONDmoNINGONMYoCARDIALISCHEM口已▲.REPERFUSIONIN.n瓜Y矾TATSAbstractObjective:ToinvestigatetheeffectofAlprostadilpostconditioningonmyoc
6、ardialischemia-referfusioninjuryinrats.Methods:Theratmodelsofmyocardialischemia—reperfusionwasestablishedwithocclusionoftheleftanteriordescendingcoronaryartery(LAD)for30minandreperfusionfor60min.32ratsweredividedinto4groups,groupAofthemwastakenasshamoperationgroup,groupBcontr
7、olgroup(ischemia30min,reperfusion60min),groupC(Alprostadil0.5}tg/kg,iv,5minpriortoreperfusion),andgroupD(Alprostadil1I_tg/kg,iv,5minpriortoreperfusion).ThechangesofhemodynamicsparameterandserumcardiactropininI(cTnI)weredetected.Thepathologicalchangesofmyocardiumwereobservedwi
8、thmicroscopeandtransmissionelectronmicroscope.Theheartswereremovedfo
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