阻断吡娜地尔后处理大鼠心肌线粒体比较蛋白质组学的分析

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1、遵义医学院硕士学位论文阻断吡那地尔后处理大鼠心肌线粒体比较蛋白质组学的研究reliabilityof2一DE．Results：1．ThepurifiedmyocardialmitochondriaisolatedbyNycodenzdensitygradientcentrifugationwereprovedquitewellthroughwesternblotanalysis．2．Thecardiacfunction：Therewasnoevidentchangeeachotherattheendpointofequilibration．Afterreperf

2、usion，GroupNorandGroupPinawasobviouslybetterthanGroupI／Rand5HD+Pina(P<0．05)．TherewasnosignificantdifferencebetweenGroup5HD+PinaandGroupI／R．3．Obtainedwellfocuseanddistinct2-DEmaps，meansof485+22proteinspotsweredetectedineachgroup．Therewere9proteinspotsthattheirdifferenceexceed2times，an

3、dthesedifferentialexpressionproteinsweresuccessfullyidentifiedbyMS．4．TheexpressionlevelsofATPAandNDUFA10weredecreased，whilethoseofACADL，NDUFS2，NDUFA10，ALDH2，ODP2andIMMTwereincreasedinGroup5-HD+PinacomparedwithGroupPina．Interestingly,threespotsinthe2-DEmapwereidentifiedasNDUFAl0．5．Wes

4、ternblotverification；TheexpressionofNDUFAl0，NDUFS2andALDH2areincreased，buttheATPAisdecreasedinGroup5HD+Pina．Conlusion：1．Pinacidilpostconditioningcanobviouslyimprovetheratheartfunction，whiletheeffectioncanbeantagonizedby5-hydroxydecanoate．．2．5-HDantagonizepinacidilpostconditioningbloc

5、kthemitochondrialATP—sensitivepotassiumchannel，probablyleadingtothefollowingresults：①TheexpressionlevelsofATPAisdecreasedafter5HD+Pin乱whichisrelatedtotheabnormalsynthesisandtransportprocesses．②TheexpressionofACADL，NDUFS2，ALDH2，ODP2andIMMTareincreased，whichcanresultfromthecompensatory

6、mechanism．③NDUFAIOmaybeshearedormodifiedwhenmitochondrialATP—sensitivepotassiumchannelwasantagonized．3．UtilizingNycodenzdensitygradientcentrifugationafterdifferentialcentrifugationcanobtainmyocardialmitochondriawithhigherpurity．KeyWords：5-hydroxydecanote：Pinacidil；Postconditioning；Mi

7、tochondria；Proteomics：Two—dimensionalelectrophoresis；Myocardium——●、——遵义医学院硕士学位论文阻断吡那地尔后处理大鼠心肌线粒体比较蛋白质组学的研究．iL—．L目IJ吾缺血再灌注损伤(ischemicreperfusioninjury，I／R)是当今心肌保护研究的热点，近年来集中于通过缺血后处理(ischemicpostconditioning，IPTC)以激发机体内源性抗再灌注损伤能力。Staat[1]首次将缺血后处理应用于临床冠脉成形术来治疗进行性急性心肌缺血，收到了较好的心脏保护作用。然而，缺

8、血后处理存在着反复嵌闭血