灵芝多糖肽对大鼠心肌细胞缺血再灌注损伤的保护作用

《灵芝多糖肽对大鼠心肌细胞缺血再灌注损伤的保护作用》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、分类号：R96学校代码：10392学科专业代码：100706学号：200901498福建医科大学硕士研究生毕业论文灵芝多糖肽对大鼠心肌细胞缺血再灌注损伤的保护作用ProtectiveEffectsofGanodermaLucidumPolysaccharidesPeptideonMyocardialIschemicReperfusionInjuryintheRats学位类型：医学硕士所在学院：药学院研究生：蒋丹容学科、专业：药理学导师：游育红教授研究起止日期：2010年9月至2012年3月答辩日期：2012年

2、6月8日二○一二年六月万方数据万方数据目录一、主要英文缩略语···············································1二、中文摘要·····················································2三、英文摘要·····················································6四、正文·····················································10

3、前言·····················································101﹒实验材料·····················································122﹒实验方法·····················································133﹒实验结果·····················································154﹒讨论·············

4、········································295﹒结论·····················································32五、参考文献·····················································33六、致谢·····················································35七、文献综述······························

5、·······················36万方数据主要英文缩略语GLPPGanodermalucidumpolysaccharides灵芝多糖肽peptideHRheartrate心率+dT/dtmaxPeaksystolicvelocity最大收缩速度-dT/dtmaxPeakdiastolevelocity最大舒张速度CFCoronaryflow冠脉流量Minminute分钟TUNELTdT-mediateddUTP-biotinnickend原位末端标记法labelingPBSphosphate

6、buffersolution磷酸盐缓冲液DABdiaminobenzidine二氨基联苯胺SDSsodiumdodicylsulfate十二烷基磺酸钠PAGEpolyacrylamidegelelectrophroesis聚丙烯酰胺凝胶电泳ROSreactiveoxygenspecies活性氧1万方数据灵芝多糖肽对大鼠心肌细胞缺血再灌注损伤的保护作用中文摘要目的：建立大鼠离体心肌缺血再灌注模型，探讨灵芝多糖肽对离体心肌缺血再灌注的保护作用及机理。方法：雄性SD大鼠42只，取心脏Langendorff法离体灌流

7、，实验分七组（n=6）:①正常组：修正洛氏液连续灌流110min；②模型组：修正洛氏液稳定灌流20min后停灌30min，复灌修正洛氏液60min；③GLPP给药组：修正洛氏液稳定灌流20min后停灌30min，复灌液中分别加入不同浓度的GLPP（6.25、12.5、25、-1-150、100mg·L）灌流60min。实验过程实时监测心率（HR，min）、心脏-1张力（Tmin,g）、收缩幅度（△T，g）、最大收缩速度（+dT/dtmax，g·s）、最-1-1大舒张速度（-dT/dtmax,g·s）、冠脉流量

8、（CF,d·min）反应心功能。实验结束，留取心室肌光镜观察心肌组织学结构、电镜观察心肌细胞超微结构,TUNEL检测心肌细胞凋亡，Westernblot检测心肌细胞Caspase-3的活性。结果：1心搏曲线在心脏缺血再灌注期间，模型组收缩力减弱，心率减慢，出现心律失常；给药组收缩力增强，心率增快，节律整齐。2各项参数2.1心肌张力模型组在缺血20min后张力增加，对照组和模型组在缺血20min时的张