sea0400对大鼠心肌细胞缺血再灌注损伤的保护作用

《sea0400对大鼠心肌细胞缺血再灌注损伤的保护作用》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、重庆医科大学硕士研究生学位论文PRoTECTIVEEFI咂CTSoFSEA0400oNISCHEⅣⅡA，REPERFUSIoNCARDIo删oCYTESoFObjective：ISoLATEDRATHEARTToinvestigatetheprotectiveeffectsofSEA0400(2-[4一C(2，5一dinuorophenyl)methoxy]．5．ethoxyaniline)，anewlysynthesizedNa+／Ca2+exchangerinhibitor，onischemia／reperfusioncardiomyocyteso

2、fisolatedratheart．Methods：IsolatedcardiomyocyteswereobtainedfromadultratheartsbyLangendorffperfusionandenzymaticdissociation，andrandomlydividedintothreegroupsincludingnormalgroup，ischemia／reperfusioninjurygroup(I／Rgroup)andSEA0400interventiongroup．Thenormalgroupwasincubatedinnor

3、malconditionfor60minutesasacontrolgroup．TheI／Rgroupunderwentischemiafor30minutesandreperfusionfor30minutesrespectively．SEA0400interventiongroupincluded3subgroupswithSEA0400atdifferentconcentrations(0．1gmol／L，1grnol／L，109rnol／L)，andthenunderwentischemiafor30minutesandreperfusionf

4、or30minutesMTTmetholdwasappliedforthemeasurementofthecardiomyocyteviabilityattheendofreperfusionperiod．TheintracellularCa2+5重庆医科大学硕士研究生学位论文concentrationweremeasuredbylaserscanningconfocalmicroscope(LSCM)andexpressedasfluorescenceintensityofFluo一3．TheprotectiveeffectsofSEA0400atd

5、ifferentconcentrationswerestudied．Results：1．Thecardiomyocyteviabilityineachgroupdecreasedsignificantlyattheendofreperfusionperiod(p<0．01)．ComparedwithI／Rgroup，thecardiomyocyteviabilityinSEA0400interventiongroupsincreasedsignificantlyattheendofreperfusionperiod(p<0．01)．Thecardiom

6、yocyteviabilitywasmoreactiveat1gmol／Lthanthatat0．1Ⅲ'nol／LinSEA0400interventiongroup(p<0．01)．therewasnodifferencebetween19mol／Land109mol／LinSEA0400interventiongroupinthecardiomyocyteviability(p>O．05)．1pmol／LSEA0400wastheoptimalconcentrationfortheprotectionofcardiomyocyteviability

7、2．Therewasnodifferencebetweenpre—ischemiaandpost—reperfusioninthefluorescenceintensityofFluo一3innormalgroup(p>0．05)．ThefluorescenceintensityofFluo一3inI／Rgroupbegantoincreaseduringischemiaperiodandincreasedsignificantlyduringreperfusionperiod．Thechangepercentageoffluorescenceinte

8、nsityofFluo一3at1Ⅳnol／Lwassignificantlylowerthan