骨髓单个核细胞在脑缺血大鼠脑内受损区域的定向分化

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1、BoneMarrowMononuclearCellsOrientatesDifferentiationintheBrain’SImpairedRegionafterCerebralI．schemiaRatsPostgraduate：MingChenSupervisor：JianpingWangDepartmentofNeurology,TheFifthAffiliatedHospitalofZhengZhouUniversity，ZhengZhou，China，450052BackgroundandObjectivesAbst

2、ractCerebralischemicstrokeiscausedbyocclusionofthecerebralartery，leadingtofocalischaemia，destructionofneuronsandglialceils，andmotor，sensoryorcognitiveimpairments．Atpresentthemostpotentialtherapeuticstrategyforthetreatmentofischemicstrokeisthetransplantationofexogeno

3、ustotipotent／pluripotentstemcellswithself-renewalanddifferentiationcapacity．BMMNCsisaheterogeneousmixtureofcells，whichiscomposedofmesenchymalstemcells(MSCsl，hematopoieticstemcells(HSCs)，andprogenitoranddifferentiatedcells，endothelialprogenitorcells(EPCs)，eta1．Compar

4、edwithothertypesofstemcells，suchastheembryonicstemcellsandneuralstemcells，BMMNCshastheadvantagesofeasyextraction，onlyneedin91．5—3hforobtaining,withoutculturing，autologoustraIlsplantation，nomoralandethicaldisputes．Therefore，itcanberegardedasthemostidealpromisingmulti

5、potentcandidate．ThelatestresearchdemonstratesthatBMMNCsinspecificmicroenvironmentCanbeincludedtodifferentiateintocardiomyocytecells，livercells，adiposecells，Schwanncellsinvitro·AccumulatingevidenceshaveshownadministrationofBMMNCsinthetreatmentofischemiastrokeissafean

6、deffective．ThepossiblemechanismofaboveeffectsisthatitCanreleaseavarietyofneurotrophicfactorresearchafterBMMNCstransplantationaccordingtosomeresearches．However，thecriticalmechanismsarenotYetcompletelVknown，andtheBMMNCs’differentiationfateofthespecificIVAbstractmicroe

7、nvironmentaftercerebralinfarctionhasnotbeenreported．Herein，wewillfurtherevaluatebehavioralperformance，infarctvolume，andlearningandmemoryabilitiesafterintravenoustransplantationofBMMNCsinaratmodelofischemicstroke．ThenitiSfurthertodetectorienteddifferentiationofBMMNCs

8、intheimpairedfieldsincludingsub-ventricularzone(svz)，dentategyms(DG)ofhippocampusandischemic／penumbra(I／P)regioninordertoinvestigatetherap