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1、StudyontheEstablishmentandEvaluationofdeficientheatsyndromeinRatsandtheInfluenceofCommonAnemarrhenaonthisModelSpeciality:ResearchonmicrocirculationofbrainandtumorinthefieldofintegratedTCMandWesternMedicineTutor:Prof.WangShijunAuthor:CuiShulanAbstractObj
2、ective:ToestablishmethodsofinducingdeficientheatsyndromeinRatsandtoevaluatethesignsandsymptomsofthemodel,andtoobservetheinfluenceofCommonAnemarrhenaonchangesofenergymetabolismandrelatedindexsinratswithdeficientheatsyndrome.Toanalysethetendency,character
3、isticsoftheeffects.Toexploretheobjectiveindexes,thatcanreflectesColdandHeatPropertyofexperimentalanimal.Toprovideusefulknowledgeforclinicalmedicine.Methods:Inordertoinducedeficiency-heatmodelinrats,decoctionofthreeChinesemedicinalherbswithhotproperty,ac
4、onite,driedgingerandcinnamonwereadministeredintragastricallytoratsfor14days.Afterthemodelwassuccessfullyproduced,decoctionofCommonAnemarrhenawasgivenfor7daysfortreatment.Theeffectsongeneralcondition，temperature，coldandheatpreferenceindeficiency-heatmode
5、lratsafterdrugadministrationwereobserved.Collectingthefood,water,urineanddunginquantityofratsfor24hourswithmetaboliccage.TheEI,DE,FEandMEweredetectedbyOxygenHeatCalculation.T3,T4inbloodserumwereanalyzedusingEnzyme-linkedimmunosorbentassay.Fastingbloodsu
6、gar,hepaticglycogenandLDlevelsweremeasured.TheactivitiesofhepaticATPase.TheECandthecontentofATPinliverweredectedusingHPLC.Results:Ratsinmodelgrouplookedmania,weightandtoestemperaturereducedsignificantly，T3andT4levelsinbloodserumincreasesignificantly.PLS
7、analysisshownthatthesamplepointsofdeficientheatsyndromegroupandcontrolgroupwascompletelyseparated,whichwasclearlydifferentiated.AfterthetreatmentwithCommonAnemarrhena,T3andT4levelsinbloodserum,toestemperature,Urinevolum,Drinkingamountreducedsignificantl
8、y.Fastingbloodglucosesignificantlyincreased.Conclusion：DeficientheatsyndromeinRatscanbeinducedsuccessfullywithChineseherbofhotpropertyAconite,DriedgingerandCinnamon.CommonAnemarrhenacandecreaseenergymetabolismofdeficientheatsyndr
