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1、提要目的：通过观察黄连氯仿提取物、黄连水提取物对实热证大鼠体温、肝能荷、ATP酶活性、甲状腺激素含量、细胞因子活性的影响，分析其作用趋势、特点及作用规律，探寻黄连及其提取物对实热证治疗的药效机制，为临床用药提供更好的参考。方法：观察实热证大鼠给予黄连氯仿提取物、黄连水提取物进行治疗后的体温变化；采用化学比色分光光度计法检测ATP酶活力；全自动酶标仪法检测大鼠血浆T3、T4、TSH、TRH、IL-1β、IL-6、IL-8、TNF-α含量；高效液相色谱法测大鼠肝组织ATP、ADP、AMP的含量，计算肝能荷。结果：黄连氯仿提取物、黄连
2、水提取物显著降低实热证大鼠体温；能降低实热证模型大鼠的ATP酶活性和肝能荷；能抑制实热证模型大鼠下丘脑-垂体-甲状腺轴的功能；能减少实热证模型大鼠甲状腺素的分泌；对实热证模型大鼠的细胞因子活性有一定的抑制作用。结论：黄连氯仿提取物、黄连水提取物对实热证大鼠的物质代谢、能量代谢、甲状腺激素、细胞因子活性有一定的抑制作用，为今后黄连对实热证的治疗研究和临床应用提供了一定的参考价值。关键词：黄连；实热证；甲状腺；发热因子EffectsofCoptisChinensisFranchandit’sExtractinExcessiveHea
3、tSyndromeModelRatSpeciality:TraditionalChineseMateriaMedicaTutor:Prof.ZhangShaohuaAuthor:LiuChunleiAbstractObjective:Throughobservingtheeffectsofchloroformextractandwaterextractofcoptischinensisfranchonbodytemperature,hepaticenergycharge,ATPenzymeactivity,thyroidhorm
4、onelevelsandcytokineactivity,wecouldanalysisthetendency,specialityandrule,explorethepharmacodynamicmechanismtoprovidebetterreferenceforclinicalmedication.Methods:Observingthebodytemperaturechangeinexcessiveheatsyndromemodelrattreatedwithchloroformextractandwaterextra
5、ctofcoptischinensisfranch.WecouldusechemicalcolorimetryspectrophotometrictodetectATPenzymeactivity,usefullautomaticmicroplatemethodtodetectthecontentofratplasmaT3,T4,TSH,TRH,IL-1β,IL-6,IL-8,TNF-α,usehighperformanceliquidchromatographicmethodtodeterminethecontentofrat
6、livertissueATP,ADP,AMPandcalculatethehepaticenergycharge.Results:Chloroformextractandwaterextractofcoptischinensisfranchcouldreducesignificantlythebodytemperatureofexcessiveheatsyndromemodelrat,ATPenzymeactivityandhepaticenergycharge.Itcouldinhibitthefunctionofhypoth
7、alamus-pituitary-thyroidaxisofexcessiveheatsyndromemodelrat,reducethyroxinesecretionofexcessiveheatsyndromemodelratandhadacertaininhibitiononcytokineactivity.Conclusions:Chloroformextractandwaterextractofcoptischinensisfranchhadacertaininhibitiononmaterialmetabolism,
8、energymetabolism,thyroidhormoneandcytokineactivityofexcessiveheatsyndromemodelrat,whichprovidedacertainreferencevalueforthetherapyr
