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1、南京农业大学学报 2004,27(3):89～94JournalofNanjingAgriculturalUniversity杯伞发酵培养基的响应曲面法优化研究3王允祥,吕凤霞,陆兆新(南京农业大学食品科技学院,江苏南京210095)摘要:在原有的Plackett2Burman设计与实验结果基础上,采用响应曲面法(RSM)研究了影响杯伞(Clitocybesp1)AS51112生长与发酵胞外多糖的培养基关键成分的最佳水平。实验结果表明,当葡萄糖、酵母膏、胰蛋白胨、NaNO3和-1-1MgSO4分别为14100、5111、2107、2100和1128g·L时,胞外多糖最大预测值
2、为1081180μg·mL(以发酵醪计);-1-1当上述自变量为24100、3123、1152、2136和1160g·L时,菌丝最大生长量为6181mg·mL(以发酵醪计)。欲同时获得最大量的胞外多糖和菌丝量,则葡萄糖、酵母膏、胰蛋白胨、NaNO3和MgSO4须为21174、4133、1165、2145和-11150g·L,在此条件下,每毫升发酵醪可同时获得1011130μg的胞外多糖和6166mg的菌丝量。关键词:杯伞;多糖;Plackett2Burman设计;响应曲面法;中心组合设计中图分类号:Q93文献标识码:A文章编号:10002030(2004)03008906Op
3、timizationofcultivationmediumClitocybesp1AS51112fortheextracellularpolysaccharideproductionandmycelialgrowthbyresponsesurfacemethodology3WANGYun2xiang,LaFeng2xia,LUZhao2xin(CollegeofFoodScienceandTechnology,NanjingAgriculturalUniversity,Nanjing210095,China)Abstract:Basedontheresultsofaprevi
4、ousPlackett2Burmandesign,themostimportantfactorsaffectingtheyieldofextracellularpolysaccharideandmycelialgrowthofClitocybesp1AS51112insubmergedcultivationwerefurtherinvestigatedbyresponsesurfacemethodology.Five2factor,i1e.glucose,yeastextract,tryptone,NaNO3andMgSO4,five2levelfull2factorialc
5、entralcompositedesignwasemployed.Bysolvingthequadraticregressionmodelequationusingappropriatestatisticmethods,theoptimumconcentrationswere-1determined:glucose,yeastextract,tryptone,NaNO3andMgSO4were14100,5111,2107,2100and1128g·L,respectively,-1thepredictedyieldofextracellularpolysaccharidew
6、as1081180μg·mL.Whentheabovefactorswere24100,3123,1152,2136and-1-11160g·L,respectively,themaximummycelialbiomasswas6181mg·mL.Inordertoobtainthemaximumyieldofextracellularpolysaccharideandmycelialbiomassatthesametime,theoptimalconcentrationsofglucose,yeastextract,tryptone,NaNO3andMgSO4-1-1wou
7、ldbe21174,4133,1165,2145and1150g·L,respectively.Inthissituation,1011130g·mLofextracellularpolysaccha2-1rideand6166mg·mLofmycelialbiomassweregainedsimultaneously.Keywords:polysaccharide;Clitocybesp.;Plackett2Burmandesign;responsesurfacemethodology;central
