toll样受体2和4在大鼠心肌缺血2f再灌注损伤模型中的运态表达

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1、英文摘要DynamicexpressionofTolMikeReceptor2and4inaratmodelofmyocardialischemiareperfusioninjuryABSTRACTobjective：Myocardialischemic／reperfusioninjuryrMI／RI)referstoasyndromewhichwasafterhavingischemiaforsometime，myocardiumnotonlydidnotreducethedegreeofinjuryafterthebloodflowres

2、tored，butalsoaggravatedcomparedwithischemic．MI／R1wascharacterizedby10calandsystemicinflammatoryresponse．Iftheinflammationexpanded，myocardiumwouldbeinjuredandseriouslyaffectedtheleftventricularfunction．MoreandmorestudieshadshownthatMI／R1wasagroupofchronicinflammatorydiseases

3、．Nowadays，scientistsdiscoveryToll—likereceptors(TLRs)playaimportantroleatinflammationreaction．TherelationshipbetweenTLRsandinflammationbecomesmoreandmoreclosely．ToexploretheroleofTLR2andTLR4inmyocardialischemia／reperfusioninjury(MUm)byobservingthedynamicexpressionchangesatm

4、RNAandprotein1evelsearlyaftermyocardialischemia／reperfusion．Methods：Choose42malewistarratswithsimilarweight，andrandomlvdividedtheminto7groups(reperfusionforlh，2h，4h，6h，12h，24h，and7d)，eachgrouphad6rats．AnesthetizedeachratwithPentobarbitalsodium．andthenfixeditonaoperatingboar

5、d．Withthehelpofanimal’Sventilator，openingthechest，supplantingtheheart，ligationofleftanteriordescendingcoronary(LAD)，finishingtheischemicmodel．Afterischemicforlh，openthechestagain，sheartheligaturewirewithmicrosurgicalscissor，finishingthereperfusionmodel．Afterreperfusionreach

6、esdesiredtime(1，2，4，6，12，24hoursand7days)，anesthetizedtheratagain，fixedontheboard，separatedtheskinandsubcutaneoustissueofneck，exposingthetrachea，andincubated．Thenisolatedtherightcarotidandincubated．Beforecuttingofftheheart，ligationoftheca．rdiovascularagaininsitu，injectedabo

7、ut1～1．5mlphthalocyaninebluesolutionintothecarotidartery，itCallbeseenthatthe4英文摘要heartpartlyturnedblueimmediately．Cuttheheart，andputitincleanicesaline，rinsingclean，andplaceditinrefrigeratorusingacleanPetridishesforabout8min．Takentheheartoutandcutitintoslicesofabout2ram，follo

8、wedbyTTCstaining．Finally，usingascannertoscanslicesandsavethepicturesforanalysisthe